| 1. | Constitutive expression of class ii mhc is restricted to " professional " antigen - presenting cells but can be induced on various tissues by gamma interferon
Mhc -类的组成型表达仅限于“专职性”抗原提呈细胞，但细胞因子如ifn -可引起诱导型类分子表达。 |
| 2. | These results indicated that the cloned osg6b " had a constitutive characteristics but not anther specificity , and the transgene was segregated in a mendelian fashion in the tl generation
研究表明，所克隆的osg6b ’不具有花药特异性，而具有组成型表达的特征。第二部分：从大肠杆菌e |
| 3. | Analysis of acbadh expression in leaves showed that the expression levels of acbadh increased about 10 - fold after plants were treated with nacl and aba for 48 hours when compared to control plants respectively
Northernblotting杂交结果表明， badh具有组成型表达的特性。 100umaba和400mmnacl处理48小时后， badh的表达增加10倍左右。 |
| 4. | A fusion - trascriptional library was then constructed by inserting sau3ai - partially digested chromosomal dna from 7653r into bamhi cloning site of phn127 . a group of constitutive - expression fusions with different fluorescent strength were obtained
将7653r的总dna经sau3ai部分酶解并克隆到phn127上，获得的融合子库，从中筛选到gfp组成型表达的具有调控活性的dna片段。 |
| 5. | By changing pichia pastoris ' s metabolic path rationally , a constitutive recombinant p . pastoris sibas 5071 with riched sam synthetase was obtained on the basis of research of sulfide ' s metabolism in yeast in our laboratory
本实验室在对酵母细胞中含硫物质代谢研究的基础上，通过合理改造pichiapastoris的代谢通路，获得了一株组成型表达sam合成酶2 ( sams2 )的重组p . pastoris菌株sibas5071 。 |
| 6. | However , so far only the results obtained by whitelaw et al ( 2002 ) concerning leetrl gene were partly consistent with the negative model and partly against it . obviously , the action models for these ethylene receptors are far from being clearly understood
目前已经从模式植物番茄中分离了6个受体基因( leetr1 6 ) ，对它们的基因和蛋白结构特征、表达特性等已经有较多的研究，但是对组成型表达受体基因在乙烯受体系统中的功能研究较少。 |
| 7. | The promoter - probe vector phn117 in e . coli and phn127 in g " were further constructed by removing promoter while keeping sd sequence from phn115 . a teta / tetr bidirectional promoter fragment from pbr322 was respectively cloned into phn117 and phn127 and the resulted colonies were all fluorescent
并经插入pbr322上665bpteta tetr双向启动子片段后得到的转化子均发绿色荧光验证，实现了wtgfp在大肠杆菌和华癸中生根瘤菌中的组成型表达。 |
| 8. | Third , we examined the expression of ecbp21 dynamics during the incubation of angelica suspension - cultured cells and stress treatments through northern blot analysis . during the incubation of angelica suspension - cultured cells , the expression of ecbp21 gene increased from 0 to 12th day , and it reached a peak on the twelfth day . while the expression of cam gene increased from 0 to 9th day , and it reached a peak during the 6th - 9th day
3 . ecbp21表达分析: northernblot分析表明:在白芷悬浮培养细胞生长周期中， ecbp21和cam都为组成型表达， 0一12天ecbp21表达量逐渐增加，第12天达到最高，随后12一21天， ecbp21表达量稍有下降，维持在一个比较稳定状态;而o一6天时， cam表达量逐渐上升，第6一9天达到最大表达量，随后， 9一21天， cam表达量有所下降。 |
| 9. | In this dissertation , influences of various factors on plant regeneration and agrobacterium - mediated transformation of tall fescue embryogenic calli were systematically studied , and thereafter , stress tolerance - related cbf1 gene guided by constituent promoter camv 35s was incorporated into genome of this grass to obtain transgenic plants with increased stress tolerances
本论文在对高羊茅胚性愈伤组织植株再生与农杆菌介导遗传转化的多种影响因素进行系统研究的基础上，将组成型表达启动子camv35s引导的耐逆相关cbf1基因导入该草种的基因组，获得耐逆性增强的转基因植株。 |
| 10. | The plant expression vector pbin19 - rok219 was constructed , which contains cauliflower mosaic virus ( camv ) 35s promoter and noster . introduction of ibdv vp2 gene under control of camv 35s promoter resulted in the construction of binary expression vector pbr - vp2 . then the vp2 gene was in the left and right border regions , which denote the limits of the dna that is integrated into the plant genomic dna via agrobacterium fwme / ac / ms - mediated transformation
构建了表达载体pbin19 - rok219 ，以ibdv甘肃天水株的抗原基因vp2为目的基因，将vp2基因置于植物组成型表达启动子camv35s之下，构建了一个ibdvvp2基因的植物表达载体pbr - vp2 ，这样使vp2基因位于农杆菌t - dna的左右边界重复序列之间。 |
