| 1. | Then the amplified mtb8 . 4 and ms gene were subcloned into the unique nhe i and mlu i cloning sites of pci - neo expression vector
重组质粒pm 、 pms转染ps15细胞，进行稳定表达， g418筛选抗性克隆后，用rl ’ - pcr检测目的基因在mrna水平的表达。 |
| 2. | At present , intrusion detection technique is divided into anomaly detection and misuse detection . the measure of misuse detection is if the information to be dealt with matches the rule in the rule library based on known intrusive methods
目前，入侵检测技术主要分为两种：误用检测和异常检测，误用检测是根据已知的入侵手段建立一个规则库，待检测的信息与库中规则进行匹配达到检测目的。 |
| 3. | Transformation was done by electroporation . human fl extracellular domain cdna transformed to yeast host strain km71 , then his + muts phenotype transformant was screened out and cultured in flasks , and rhfl was expressed under the induction of 0 . 5 % methanol
我们提取了km71ppic9k - fl转化菌株的基因组dna进行southern实验，检测目的基因的整和插入；提取总rna ，进行了northern实验，检测fl基因在转化菌株中的表达。 |
| 4. | This new method plays an important role in the field of basic biological research , medical diagnosis and clinical therapy . because of its high - sensitive and specific interaction between macromolecules , spr biosensor is used for directly examining the reaction between targeted protein and corresponding antibody without careful purification
Spr生物传感器的高灵敏性，以及生物大j皮巴” a硕士芹仁论文v沉孟y吁”匹r ’ sn正“分子间反应的高度特异性，使不需要严格纯化而直接检测目的蛋白与抗体间的反应成为可能。 |
| 5. | After the recombinant plasmid pcdna3 . 1 / ts87 was identified by digestion of hindlll and bamh i , it transformed into cos7 by lipofectamine . expression product was identified by immunohistochemical method , sds - page and western - blot . the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3 . 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas . l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t . s artificially and sera from rabbit immunized with soluble antigen of t . s and with protein expressed by ts87 gene and by a monoclonal antibody of t . s
通过细胞的免疫组化，细胞裂解物的sds - page电泳， westem - blot分析检测目的基因的表达情况。免疫组化结果显示：重组质粒转染的细胞质中有棕褐色颗粒，而空载体转染细胞及正常细胞无此现象；细胞裂解物sds - page电泳结果显示：只有重组质粒转染的细胞在约38kd处有明显的蛋白带，这与理论计算的ts87基因表达蛋白的分子量为38kd基本一致； western - blot分析结果显示：约38kd的蛋白带能够分别被旋毛虫感染兔血清，成虫虫体可溶性抗原免疫兔血清， ts87基因原核表达蛋白免疫兔血清( ts87血清)以及一株具保护性的旋毛虫单抗特异识别。 |
| 6. | 2 . construction of chimeric mtb8 . 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8 . 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8 . 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments , respectively
3 .重组质粒在真核细胞中的表达: pm 、 pms 、 pmi和pmsl重组质粒用lipofectaminatmzo0o脂质体转染试剂转染cos一7细胞，进行瞬时表达， 48小时后，用rl ’ - pcr检测目的基因在mrna水平的表达;用westemblotting检测hil一12在蛋白质水平的表达。 |
| 7. | Basing on the result of former research , this paper synthetically analysis character of common window function and interpolation , author has conclude a few of basic principle during choosing windows function and interpolation in the process of ct image reconstruction in the terms of windows , among of the introduced windows , band - limited window , triangle window and cosine window must be the ideals choose
基于前人的研究成果，本文综合分析了常见窗函数与内插函数的特性。理论分析和实验结果得出可供选择的选择窗函数与内插函数以及如何根据实际检测目的来选择较好参函数所遵循的原则。 |
| 8. | And through simulated test with part of actural data on the computer , the author confirms that this kind of way is feasible , which estabilishs the base of designing the complete data treating system of cam ' s automatic test on the base of windowsnt
提出了凸轮轴的检测目的、检测原理及数据处理方法，并以此为根据，初步设计并编制了数据处理软件，采用部分实测数据在计算机上进行结果模拟，证实了这种处理方法的可行性，为进一步在windowsnt平台上开发完整的凸轮自动检测数据分析处理系统奠定了基础。 |
| 9. | Expression in vitro cos - 7 cells were transfected with pm , pms , pmi and pmsi constructs by cationic liposom , respectively . 48 hours later , mrna of targets gene were detected by rt - pcr and hil - 12 protein in culture supernatnant and cell lysates were detected by western blotting . p815 cells were transfected with pm and pms constructs and selected by g418
2 .重组质粒在真核细胞中的表达: ( 1 ) pm 、 pms转染cos一7细胞， 48小时后，用ri ’ - pcr检测目的基因在mrna水平的表达;转染p815细胞， g418筛选抗性细胞克隆，用rt - pcr检测目的基因在mrna水平的表达，结果为阳性，说明在转录水平有目的基因的表达。 |
