检测目的 meaning in Chinese
a test for
Examples
- Then the amplified mtb8 . 4 and ms gene were subcloned into the unique nhe i and mlu i cloning sites of pci - neo expression vector
重组质粒pm 、 pms转染ps15细胞，进行稳定表达， g418筛选抗性克隆后，用rl ’ - pcr检测目的基因在mrna水平的表达。 - At present , intrusion detection technique is divided into anomaly detection and misuse detection . the measure of misuse detection is if the information to be dealt with matches the rule in the rule library based on known intrusive methods
目前，入侵检测技术主要分为两种：误用检测和异常检测，误用检测是根据已知的入侵手段建立一个规则库，待检测的信息与库中规则进行匹配达到检测目的。 - Transformation was done by electroporation . human fl extracellular domain cdna transformed to yeast host strain km71 , then his + muts phenotype transformant was screened out and cultured in flasks , and rhfl was expressed under the induction of 0 . 5 % methanol
我们提取了km71ppic9k - fl转化菌株的基因组dna进行southern实验，检测目的基因的整和插入；提取总rna ，进行了northern实验，检测fl基因在转化菌株中的表达。 - This new method plays an important role in the field of basic biological research , medical diagnosis and clinical therapy . because of its high - sensitive and specific interaction between macromolecules , spr biosensor is used for directly examining the reaction between targeted protein and corresponding antibody without careful purification
Spr生物传感器的高灵敏性，以及生物大j皮巴” a硕士芹仁论文v沉孟y吁”匹r ’ sn正“分子间反应的高度特异性，使不需要严格纯化而直接检测目的蛋白与抗体间的反应成为可能。 - After the recombinant plasmid pcdna3 . 1 / ts87 was identified by digestion of hindlll and bamh i , it transformed into cos7 by lipofectamine . expression product was identified by immunohistochemical method , sds - page and western - blot . the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3 . 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas . l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t . s artificially and sera from rabbit immunized with soluble antigen of t . s and with protein expressed by ts87 gene and by a monoclonal antibody of t . s
通过细胞的免疫组化，细胞裂解物的sds - page电泳， westem - blot分析检测目的基因的表达情况。免疫组化结果显示：重组质粒转染的细胞质中有棕褐色颗粒，而空载体转染细胞及正常细胞无此现象；细胞裂解物sds - page电泳结果显示：只有重组质粒转染的细胞在约38kd处有明显的蛋白带，这与理论计算的ts87基因表达蛋白的分子量为38kd基本一致； western - blot分析结果显示：约38kd的蛋白带能够分别被旋毛虫感染兔血清，成虫虫体可溶性抗原免疫兔血清， ts87基因原核表达蛋白免疫兔血清( ts87血清)以及一株具保护性的旋毛虫单抗特异识别。