English translation for "限制性酶切位点"
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- restriction enzyme cutting site
Related Translations:
限制性政策: restrictive policy 限制性的: prohibitiverestrictive 限制性自行: restricted proper motion 限制性背书: restricted endorsementrestrictive endorsement 限制性效应: restrictive effect 限制性定语: restrictive attribute
- Example Sentences:
| 1. | On the base of construction of pbi121vp7 , we constructed the fusion gene expression vector pbi121ctbvp7 by the same sigle cloning site ( ndei and xbai ) of vector puc19ctb and pbi121vp7
设计具有ndei和saci单限制性酶切位点的vp7基因引物，通过pcr扩增出vp7基因并经测序验证。 | | 2. | 2 . ecori / bamhi digested pcr products were inserted into the corresponding restriction site in the expression plasmid pbv220 . construction of non - fusion expression plasmid pbv220 - endostatin
用ecori和bamhi酶切endostatincdna的pcr产物，将其插入到质粒载体pbv220中相应的限制性酶切位点，构建非融合质粒表达载体pbv220 - endostatin 。 | | 3. | The results indicated that : ( 1 ) marinated in 1 % agno3 for 15min was the optimal sterile condition of seeds . ( 2 ) 2 , 4 - d had great effects on the formation of callus , and the optimal concentration was 0 . 1mg / l
在pbi121vp7表达载体成功构建的基础上，利用质粒pbi121vp7和质粒puc19ctb上相同的ndei和xbai单限制性酶切位点，构建了pbi121ctbvp7融合表达载体。 | | 4. | It has made the strong basis for further studying mechanism of replication , virulence and determinant , attenuation , pathogenesis , functions of genetic products , specific diagnosis , cell and host tropism , development of dna vaccine and marker vaccine of csfv , and provided an excellent tool for molecular virology . main research contents include : based on published nucleotide sequences of csfv and by the help of computer analysis software , high conservative regions and single restriction enzyme sites of genome were selected . utilizing rt - pcr and nested - pcr techniques , 7 overlapping cdna fragments covering the full genome of csfv c - strain were successfully amplified
中国猪瘟兔化毒(脾淋毒)基因组cdna文库的构建、序列分析：根据已发表的猪瘟病毒( csfv )核苷酸序列，借助计算机软件分析，选择高保守区段和基因组中的单一限制性酶切位点，利用rt - pcr及nested - pcr和helf - nestedpcr技术，成功地扩增出了覆盖c -株全基因组的7个cdna重叠片段f1 f7 ，分别克隆到pmd - 18t或pgem - teasy载体进行测序后，拼接出了其核苷酸序列。 |
- Similar Words：
- "限制性联想" English translation, "限制性流动" English translation, "限制性马拉色菌" English translation, "限制性酶切片段长度多态性" English translation, "限制性酶切图" English translation, "限制性酶位点引入pcr" English translation, "限制性内切核酸酶" English translation, "限制性内切核酸酶图谱" English translation, "限制性内切核酸酶作图" English translation, "限制性内切酶" English translation
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