限制性酶切位点 meaning in English
restriction enzyme cutting site
Examples
- On the base of construction of pbi121vp7 , we constructed the fusion gene expression vector pbi121ctbvp7 by the same sigle cloning site ( ndei and xbai ) of vector puc19ctb and pbi121vp7
设计具有ndei和saci单限制性酶切位点的vp7基因引物，通过pcr扩增出vp7基因并经测序验证。 - 2 . ecori / bamhi digested pcr products were inserted into the corresponding restriction site in the expression plasmid pbv220 . construction of non - fusion expression plasmid pbv220 - endostatin
用ecori和bamhi酶切endostatincdna的pcr产物，将其插入到质粒载体pbv220中相应的限制性酶切位点，构建非融合质粒表达载体pbv220 - endostatin 。 - The results indicated that : ( 1 ) marinated in 1 % agno3 for 15min was the optimal sterile condition of seeds . ( 2 ) 2 , 4 - d had great effects on the formation of callus , and the optimal concentration was 0 . 1mg / l
在pbi121vp7表达载体成功构建的基础上，利用质粒pbi121vp7和质粒puc19ctb上相同的ndei和xbai单限制性酶切位点，构建了pbi121ctbvp7融合表达载体。 - It has made the strong basis for further studying mechanism of replication , virulence and determinant , attenuation , pathogenesis , functions of genetic products , specific diagnosis , cell and host tropism , development of dna vaccine and marker vaccine of csfv , and provided an excellent tool for molecular virology . main research contents include : based on published nucleotide sequences of csfv and by the help of computer analysis software , high conservative regions and single restriction enzyme sites of genome were selected . utilizing rt - pcr and nested - pcr techniques , 7 overlapping cdna fragments covering the full genome of csfv c - strain were successfully amplified
中国猪瘟兔化毒(脾淋毒)基因组cdna文库的构建、序列分析：根据已发表的猪瘟病毒( csfv )核苷酸序列，借助计算机软件分析，选择高保守区段和基因组中的单一限制性酶切位点，利用rt - pcr及nested - pcr和helf - nestedpcr技术，成功地扩增出了覆盖c -株全基因组的7个cdna重叠片段f1 f7 ，分别克隆到pmd - 18t或pgem - teasy载体进行测序后，拼接出了其核苷酸序列。