| 1. | Using ribosome scanning model to predict translation initiation sites
用核糖体扫描模型预测翻译起始位点 |
| 2. | Tis , translation initiation site
真核生物翻译起始位点 |
| 3. | Computational location of transcription start sites in prokaryotic genome based on sliding window
基于滑动窗口的原核转录起始位点计算定位方法 |
| 4. | By artificially changing a to c at - 137 bp site upstream from transcription start point of cloned promoter , two site - mutation promoters , ipms ( 603 bp ) and ipm1 ( 900 bp ) were created
通过pcr方法对克隆的两个启动子进行定点突变，使转录起始位点上游- 137bp处a突变为c ，得到两个突变启动子( ipml 、 ipms ) 。 |
| 5. | Uppsala university scientists have discovered an entirely new process in which short , tiny " antisense rna " competes with the protein - producing ribosomes for starting sites for reading messenger rna
乌普萨那大学的科学家们发现了一个全新的进程，在短期内，微小的“反义核糖核酸”具有与制造蛋白质的核糖体竞争信使rna起始位点的能力。 |
| 6. | Activity of each construct was determined . the basal promoter was located at about 60bp up stream of the transcription initiation site . it contains a tata box at - 33bp which is required for the transcription initiation
基因的基本启动子元件位于转录起始位点上游约60bp ，其中含有一个位于- 33bp处的tatabox ，它对于转录起始起着重要作用。 |
| 7. | 900 bp promoter - directed gus expression was highly induced by sa and bth , while the 603 bp promoter , whether mutated or not , did not respond to sa and bth induction , which indicated that the element in response to sa and bth lied among 575 ~ 872 bp from transcription start site
全长900bp启动子能够应答sa和bth的诱导，而603bp长的启动子无论突变与否对sa和bth均无应答，证明sa和bth的应答区域在克隆启动子的转录起始位点上游575 872bp之间。 |
| 8. | The pa7 fragment was sequenced and several motifs similar to prokaryotic promoter elements such as - lobox , - 35box and up box were found . the sd sequence which was bound by ribosome and atg site were also found . the pat fragment was blast in genebank , but there is no its " homological sequence
对pa7片段的序列分析发现其距5 ’端931bp ? 1091bp处具有原核生物典型基因启动子的保守结构- 10区和- 35区，以及翻译必需的sd序列和翻译起始位点等。 |
| 9. | In order to use the responding ability to the inducers of pr - la promoter , two fragments , ip1 ( 900 bp ) and ips ( 603 bp ) were cloned from tobacco genomic dna by polymerase chain reaction ( pcr ) with primers designed according to the sequence reported in genbank . sequence analysis of the amplified 900 bp fragment indicated that the cloned sequence had 99 % of homology to the known sequence . its transcription start site , tata box and consensus sequence " tgac " conserved in pr genes were identical to those of pr - la promoter
根据pr - 1a基因的报道序列，设计两对引物，以烟草基因组为模板，通过pcr扩增得到900bp ( ipl )和603bp ( ips )两个目标片段，序列测定表明克隆的启动子与报道序列具有99的同源性，转录起始位点、 tatabox及保守序列tgac与报道序列均完全相同。 |
| 10. | The entire halcs gene is located on a megaplasmid and contains a 849 - bp open reading frame that encodes a polypeptide of 283 amino acids . the promoter is typically haloarchaeal , but the start site of transcription is only seven bases from the 5 ' end of the initiator aug codon , making the halcs transcript another example of a " leadless transcript " in the haloarchaea
它的一29一24位置上是一“毛幻人” box序列，为典型的嗜盐古菌启动子;它的转录起始位点距离“ atg ”只有7个核普酸，也是典型的嗜盐古菌的“卜adless腼script ’ ’ 。 |
