| 1. | Computational location of transcription start sites in prokaryotic genome based on sliding window
基于滑动窗口的原核转录起始位点计算定位方法 |
| 2. | Protein expression could be regulated at several levels . however , the most important one is at the transcriptional initiation
蛋白质表达可以在多个水平受到调控，但是最重要的调控发生于转录起始阶段。 |
| 3. | Promotor , in broad sense , consists of transcription start site , binding site of rna polymerase ( promoter in narrow sense ) and upstream regulation sequence
广义的启动子（ promotor ）包含有转录起始部位、 rna聚合酶与dna结合部位（也就是狭义的启动子）以及上游调控序列。 |
| 4. | Through double - labeling immunoelectron microscopy , the same pattern was found as that of the above immunofluoresent microscopic data . 4 , it has been showed that actin is a subunit of baf complex
Nn几tf可能是通过与brgi和rna聚合酶h小亚基b6的联系参与了转录起始复合物的形成。 |
| 5. | By artificially changing a to c at - 137 bp site upstream from transcription start point of cloned promoter , two site - mutation promoters , ipms ( 603 bp ) and ipm1 ( 900 bp ) were created
通过pcr方法对克隆的两个启动子进行定点突变，使转录起始位点上游- 137bp处a突变为c ，得到两个突变启动子( ipml 、 ipms ) 。 |
| 6. | 2 . specific mutation from a to c at - 137 bp site upstream from transcription start point had no effect on the inducibility of the promoter in response to sa and bth . 3
在转录起始点上游137bp定点将a突变为c后，对化学启动子的化学诱导应答性没有明显的影响，但对诱导效应的向上运输存在一定程度的阻抑作用。 |
| 7. | A late baculoviral transcription initiation motif ataag was found 65 nt upstream of the putative translational start site and a polyadenylation signal aataaa was identified 14 nt downstream of the taa stop codon
在其起始密码子上游- 65nt发现杆状病毒晚期启动子转录起始信号ataag ，在其终止密码子下游- 14nt发现polya加尾信号aataaa 。 |
| 8. | Activity of each construct was determined . the basal promoter was located at about 60bp up stream of the transcription initiation site . it contains a tata box at - 33bp which is required for the transcription initiation
基因的基本启动子元件位于转录起始位点上游约60bp ，其中含有一个位于- 33bp处的tatabox ，它对于转录起始起着重要作用。 |
| 9. | A late transcriptional motif , ataag , was found at - 50nt upstream of the translational start codon atg , while two tata box was located at - 112nt and - 189nt upstream of atg . a typical poly ( a ) signal was found at 12nt downstream of the translational stop codon
在hel起始密码子atg上游50位有强晚期启动子转录起始信号ataag ，在? 112位和? 189位存在两个tatabox ，但未发现早期转录信号cagt 。 |
| 10. | The ie 180 mutant combined with two special protein binding - sites and inhibited the promoter transcription . accidentally in the approach showed that the plasimd pcdna3 show as activator to sv40 and cmv early promoter . the result is acquired by instantaneous transinfect
这说明不同的ie180突变体，对于sv40启动于这类在转录起始位置一侧只有一个“ 5 ’ atcgt 3 ’ ”特征蛋白质结合序列的11类基因启动于，可分别表现出抑制活性与激活性。 |
