Fruit and vegetable juices . enzymatic determination of l - matic acid l - malate content . nadh spectrometric method 水果和蔬菜汁. l -苹果酸含量的酶催化的测定. nadh光谱法
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Method for enzymatic determination of l - malic acid l - malate content of fruit and vegetable juices - nadh spectrometric method 果汁和蔬菜汁中l -苹果酸的酶催化测定法. nadh光谱法
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Fruit and vegetable juices - enzymatic determination of l - malic acid l - malate content - nadh spectrometric method ; german version en 1138 : 1994 水果和蔬菜汁. l苹果酸含量的酶性测定. nadn光谱测定
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We present a preliminary study on the zymograms of esterase ( est ) , lactate dehydrogenase ( ldh ) , malate dehydrogenase ( mdh ) from four groups of microtus fortis by vertical discontinous polyacrylamide gel electrophoresis method . the number , mobility and dye intensity of the enzyme band were analyzed 采用垂直板不连续聚丙烯酰胺凝胶电泳法对四类东方田鼠的酯酶( est ) 、苹果酸脱氢酶( mdh )和乳酸脱氢酶( ldh )进行了初步研究。
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First , three isozymic systems ( lactate dehydrogenase ( ldh ) , esteraes ( est ) , malate dehydrogenase ( mdh ) ) extracted from threepopulations of mandarinfishes were detected by discontinuous vertical plate polyacrylamide gel electrophoresis ( page ) . several loci were tested but none polymorphic locus was detected in qiupu river population . the results showed that : ldh can be used as biochemical markers to identify these three populations of mandarinfishes 首先,本试验采用聚丙烯酰胺凝胶电泳( page )对我省三个水域鳜鱼群体3种同工酶( ldh 、 est 、 mdh )的不同基因座位进行了检测,结果表明: ldh同工酶可以作为区分秋浦河鳜鱼、长江鳜鱼两个群体与万佛湖鳜鱼群体的生化遗传标记;其次,运用rapd技术分析了三群体鳜鱼的基因组dna的多态性。
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The tested isozymes included esterase ( est ) 、 superoxide dismutase ( sod ) 、 malate . dehydrogenase ( mdh ) 、 malic enzyme ( me ) 、 glutamate dehydrogenase ( gdh ) 、 peroxidase ( per ) and alcohol dehydrogenase ( adh ) . there were obvious differences between the normal strain and its degenerative strain in est 、 mdh 、 me 、 sod . the zymotype were the same in the other isozymes . the results were not only used to test the degenerative strain at young stage , but also as the theoretical base to study the genetic mechanism of the degenerative strain 而其余四种酶在正常菌株和退化菌株中酶谱一致。本研究不仅可以作为菌株退化早期鉴别的手段之一,而且为进一步研究菌种退化遗传机理提供了蛋白质水平上的一些理论依据。