English translation for "kozak"
|
- 科扎克
克柴
Related Translations:
- Example Sentences:
| 1. | Shilah phillips , the first black miss texas , was first runner - up , and miss georgia , amanda kozak , was second runner - up
“美国小姐”的亚军和季军分别是来自得克萨斯州的史拉?菲利普和来自佐治亚州的阿曼达?柯萨克。 | | 2. | Plant mutual sequence of starting translation and kozak sequence in animals have the same function namely accelerate translation starting of mrna
植物翻译起始共有序列与动物体中的kozak序列具有相同的功能，能够加速mrna的翻译起始。 | | 3. | Ncbi blastn analysis indicated that similarity of hsa cdna sequence and the postulated ammonic acids sequence is 99 . 6 % and 99 . 5 % , compared with the reported sequence in genbank . so the modified hsa cdna with kozak sequence was obtained
Ncbiblastn分析的结果表明，获得的hsacdna序列及推导的氨基酸序列与genbank中所报道的序列的同源率分别为99 . 6 、 99 . 5 。 | | 4. | At the same time , vp4 gene was mutated in a certain point by pcr . the plant expression vector : pbi121 was constructed and then transformed to agrobacteriutn tumefaciens eha105 directly . a . tumefaciens eha105 harboring pbi121 / vp4 was used to transform the boechmeria nivea l . guad according to the leaf disc procedure
为便于基因操作，对外壳抗原蛋白vp4基因进行适当的修饰和改造：通过引物设计，利用pcr反应，在基因的起始编码前引入有助于真核生物表达的kozak序列和限制性内切酶位点；使用套叠pcr对vp4基因进行定点突变，以便于将改造后的基因插入pbi121构建植物表达载体，通过直接转化法，把pbi121 vp4转入农杆菌eha105 ，构建了农杆菌工程菌。 | | 5. | In this research , total rna was extracted from fetal liver by the modified single - step method of acid guanidinium thiocyanate - phenol - chloroform ( agpc ) . based on reported sequence of hsa cdna , specific primers were designed to amplify its encoding region by rt - pcr . at the same time , kozak sequence was added to the translation site , to strengthen initiation of translation
本研究通过改进的异硫氰酸胍-酸性酚-氯仿一步法从人工流产胎儿肝脏中提取总rna ，然后根据报道的序列设计特异性引物，同时在上游引物中引入了kozak序列，经rt - pcr克隆到hsacdna编码区序列，长度为1758bp 。 | | 6. | In order to construct the recombinant plasmid pcdna3 . 1 / ts87 , first , the ts87gene fragment was repcred using the redesigned primers to introduce re sites of hindld and bamh i , and kozak sequence . then the rebuilt ts87 fragment was cloned into t - vector and transformed into e . coli dh5 a
通过重新设计引物在ts87两端加上用于构建重组质粒的酶切位点hind和bamh和用于真核表达的kozak序列。将改造后的基因片段克隆入t - vector ，转化大肠杆菌dh5 ，通过蓝白斑筛选获得阳性克隆后测序鉴定。 | | 7. | Ln our experiment , a lot of regulator sequences inc1uding the 35s promoter with double enhancer e1ements and the terminator nos , the fl sequence and kozak sequence for improving the genetic statement in the level of translation , were appended on the two s ides of the artifically pseudopleuronectes americanus antifreeze protei n gene by a series of process , and the products were linked into pbil21
本实验中人工合成了美洲拟鲽抗冻蛋白基因，并在美洲拟鲽抗冻蛋白基因两侧添加了许多调控序列，如具有加倍增强子的35s启动子和nos终止子，它们可以促进抗冻蛋白基因的转录，及可在翻译水平上提高基因表达的序列和kozak序列，从而构建了抗冻蛋白基因的高效表达盒。 | | 8. | Is toxic to tenebrio molitor l . . the homological cry3aa gene was modified according to the characters of poplus genes and the other features of eukaryotic gene , and was artificially synthesized . the original and modified homological c / y3aa gene were cloned into binary vector pb121 and pbin438 respectively , and transplanted into 84k clone ( p . tomentos x p . glandulossa ) to breed new anti - insects tree species
通过对氨基酸密码子的偏好、使用频率、多聚腺苷酸信号序列( ppss ) 、 mrna不稳定序列( dst )以及kozak等结构的优化改造，人工设计、合成了预期能在杨树中高效表达的毒蛋白基因modifiedcry3aa同源基因； ( 4 )利用强启动子表达载体pet30 - b完成了cry3aa同源基因在大肠杆菌中的表达。 |
- Similar Words：
- "kozaemon" English translation, "kozagaci" English translation, "kozagawa, wakayama" English translation, "kozai" English translation, "kozaicevski" English translation, "kozak jaroslav" English translation, "kozaka" English translation, "kozakai" English translation, "kozakai, aichi" English translation, "kozakevic" English translation
|
|
|
