kozak meaning in English
科扎克
克柴
Examples
- Shilah phillips , the first black miss texas , was first runner - up , and miss georgia , amanda kozak , was second runner - up
“美国小姐”的亚军和季军分别是来自得克萨斯州的史拉?菲利普和来自佐治亚州的阿曼达?柯萨克。 - Plant mutual sequence of starting translation and kozak sequence in animals have the same function namely accelerate translation starting of mrna
植物翻译起始共有序列与动物体中的kozak序列具有相同的功能,能够加速mrna的翻译起始。 - Ncbi blastn analysis indicated that similarity of hsa cdna sequence and the postulated ammonic acids sequence is 99 . 6 % and 99 . 5 % , compared with the reported sequence in genbank . so the modified hsa cdna with kozak sequence was obtained
Ncbiblastn分析的结果表明,获得的hsacdna序列及推导的氨基酸序列与genbank中所报道的序列的同源率分别为99 . 6 、 99 . 5 。 - At the same time , vp4 gene was mutated in a certain point by pcr . the plant expression vector : pbi121 was constructed and then transformed to agrobacteriutn tumefaciens eha105 directly . a . tumefaciens eha105 harboring pbi121 / vp4 was used to transform the boechmeria nivea l . guad according to the leaf disc procedure
为便于基因操作,对外壳抗原蛋白vp4基因进行适当的修饰和改造:通过引物设计,利用pcr反应,在基因的起始编码前引入有助于真核生物表达的kozak序列和限制性内切酶位点;使用套叠pcr对vp4基因进行定点突变,以便于将改造后的基因插入pbi121构建植物表达载体,通过直接转化法,把pbi121 vp4转入农杆菌eha105 ,构建了农杆菌工程菌。 - In this research , total rna was extracted from fetal liver by the modified single - step method of acid guanidinium thiocyanate - phenol - chloroform ( agpc ) . based on reported sequence of hsa cdna , specific primers were designed to amplify its encoding region by rt - pcr . at the same time , kozak sequence was added to the translation site , to strengthen initiation of translation
本研究通过改进的异硫氰酸胍-酸性酚-氯仿一步法从人工流产胎儿肝脏中提取总rna ,然后根据报道的序列设计特异性引物,同时在上游引物中引入了kozak序列,经rt - pcr克隆到hsacdna编码区序列,长度为1758bp 。