English translation for "ccv"

犬冠状病毒
犬冠状簿

Related Translations:

ccv craig cove:  克雷格考夫

ccv coal corof victoria:  维多利亚煤炭公司

Example Sentences:

	1.	Ccv core cavity design 阴阳模设计
	2.	In trpsin tolerance assay . this virus could resist to 1 % trpsis at 37 in an hour . in acid tolerance assay , this virus was resistant to ph3 . 0 and ph5 . 0 at 37 in 2 hours , and the average infection litre of the virus decreased little . in heat assay , at 50 , the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree . among these conditions , when at 50 in 30 minutes . the average infection litre of this virus decreased over 2 tilre . and when al 50 in an hour , cpe of ihis virus disappeared . when time was set for an hour . but with processed in different temperature as 50 60 70 , 80 , the virus losl the multiplication capacity complelely . in biological assay , we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory . then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second . with f81 cell line , after passaged for 12 times continuously with low concentration of fcs . the virus could produce cpe . however , with vero cell line . the virus could not procuce any cpe after many passages . the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig , fowl and cavy . by neutrolizaion assay , dxmv could be identified as a kind of ccv 理化学研究表明，该病毒为rna病毒，对氯仿、乙醚敏感；胰酶试验中，经37 、 1小时处理的病毒，仍然能够在猫源细胞fcwf细胞上生长，并且毒力基本保持不变；耐酸性试验中，病毒分别在ph5 . 0和ph3 . 0经37作用2小时，毒力仅下降一个滴度；耐热性试验中，该病毒在恒定温度50 ，设定不同时间，从5分钟到150分钟，毒力均有不同程度下降，其中， 50作用30分钟，病毒平均滴度下降2个单位； 50 ， 60分钟， cpe消失；恒定时间1小时，设定不同温度( 50 - 60 - 70 - 80 ) ，病毒在细胞上完全丧失增殖能力， cpe消失。生物学试验，利用实验室现有条件，选择不同的细胞系对该病毒进行培养，发现该病毒对猫源细胞fcwf最敏感； mdck细胞次之； f81细胞经多次传代，亦可出现cpe ；而vero细胞则不敏感。血凝试验表明，该病毒对猪、鸡、人及豚鼠的红细胞均无血凝性。
	3.	In order to identifiy the virus further , a set of double nested primers for canine coronavirus was selected . the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl , ccvf2 - ccvr2 . the first is a pair of outer primer , and can amplify a fragement of 1086bp . the second is a pair of inner primer . and can amplify a fragement of 515bp . using the nested primers , many ccv strains can be identificated including k378 , insave - l , ccv 1 - 71 etc . synthesizing this set of primers , we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr , and all of them respectively gained two target fragements of 1086bp and 515bp , but the control cell did not 合成该套式引物，选择大熊猫原代病料和病毒各代细胞培养物，经套式( nested ) rt一pcr扩增，可得到一与设计值5巧bp相符的dna片段，经bst一xl ( 590 ， 1110 )酶切鉴定，证明该扩增片断为特异性片段;回收大熊猫肝组织原代病料和细胞培养物第2 、 3 、 29代的ccvfz一ccvrz扩增片段，纯化，送生物公司测序。

Similar Words：

"ccurrence" English translation, "ccurrent page" English translation, "ccurved stereotype shaving machine" English translation, "ccus" English translation, "ccustomer premise" English translation, "ccv coal corof victoria" English translation, "ccv craig cove" English translation, "ccv-av" English translation, "ccva" English translation, "ccvd" English translation