ccv meaning in English
犬冠状病毒
犬冠状簿
Examples
- Ccv core cavity design
阴阳模设计 - In trpsin tolerance assay . this virus could resist to 1 % trpsis at 37 in an hour . in acid tolerance assay , this virus was resistant to ph3 . 0 and ph5 . 0 at 37 in 2 hours , and the average infection litre of the virus decreased little . in heat assay , at 50 , the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree . among these conditions , when at 50 in 30 minutes . the average infection litre of this virus decreased over 2 tilre . and when al 50 in an hour , cpe of ihis virus disappeared . when time was set for an hour . but with processed in different temperature as 50 60 70 , 80 , the virus losl the multiplication capacity complelely . in biological assay , we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory . then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second . with f81 cell line , after passaged for 12 times continuously with low concentration of fcs . the virus could produce cpe . however , with vero cell line . the virus could not procuce any cpe after many passages . the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig , fowl and cavy . by neutrolizaion assay , dxmv could be identified as a kind of ccv
理化学研究表明，该病毒为rna病毒，对氯仿、乙醚敏感；胰酶试验中，经37 、 1小时处理的病毒，仍然能够在猫源细胞fcwf细胞上生长，并且毒力基本保持不变；耐酸性试验中，病毒分别在ph5 . 0和ph3 . 0经37作用2小时，毒力仅下降一个滴度；耐热性试验中，该病毒在恒定温度50 ，设定不同时间，从5分钟到150分钟，毒力均有不同程度下降，其中， 50作用30分钟，病毒平均滴度下降2个单位； 50 ， 60分钟， cpe消失；恒定时间1小时，设定不同温度( 50 - 60 - 70 - 80 ) ，病毒在细胞上完全丧失增殖能力， cpe消失。生物学试验，利用实验室现有条件，选择不同的细胞系对该病毒进行培养，发现该病毒对猫源细胞fcwf最敏感； mdck细胞次之； f81细胞经多次传代，亦可出现cpe ；而vero细胞则不敏感。血凝试验表明，该病毒对猪、鸡、人及豚鼠的红细胞均无血凝性。 - In order to identifiy the virus further , a set of double nested primers for canine coronavirus was selected . the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl , ccvf2 - ccvr2 . the first is a pair of outer primer , and can amplify a fragement of 1086bp . the second is a pair of inner primer . and can amplify a fragement of 515bp . using the nested primers , many ccv strains can be identificated including k378 , insave - l , ccv 1 - 71 etc . synthesizing this set of primers , we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr , and all of them respectively gained two target fragements of 1086bp and 515bp , but the control cell did not
合成该套式引物，选择大熊猫原代病料和病毒各代细胞培养物，经套式( nested ) rt一pcr扩增，可得到一与设计值5巧bp相符的dna片段，经bst一xl ( 590 ， 1110 )酶切鉴定，证明该扩增片断为特异性片段;回收大熊猫肝组织原代病料和细胞培养物第2 、 3 、 29代的ccvfz一ccvrz扩增片段，纯化，送生物公司测序。