English translation for "bgl"
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- 井眼几何形状测井
Related Translations:
- Example Sentences:
| 1. | Bgl bulgaria leva
Bgl保加利亚利瓦 | | 2. | As heterologous probe and subsequently show to code for desired enzymatic activity . after a serial of subcloning coupled with southern hybridization and enzymatic activity assay , the functional s . griseus atcc14811 cholesterol oxidase gene ( chog ) was localized onto 2 . 3kb ecori - sall fragment
对phz1140和phz1141进行bamh及bgl的酶谱分析及与choa探针的杂交，将胆同醇氧化酶基因初步定位在8 . 8kbbamh和9 . 9kbbgl片段上。 | | 3. | Based on the restriction endonuclease sites of the reference sequences of a , b , e subgroups published in ncbi , the dna was digested by restriction endonucleases bgiii , bamhi , sspi . the expected results were obtained when digested by bgiii specific to a subgroup or sspi specific to a and e subgroups
结果发现，扩增到的目的带只能用对a亚群特异的bgl限制酶或对a 、 e亚群特异的ssp限制酶切开，而不能用对b亚群特异的bamh限制酶切开。 | | 4. | Though the expression level of bl21 ( de3 ) / pt221 - hyuh was lower than that of m15 / pqe60 - hyuh , the target protein of bl21 ( de3 ) / pt221 - hyuh was principally in soluble form while the objective protein of m15 / pqe60 - hyuh was principally in insoluble form . both of the products in the two strains showed biological activity , but the former is 2 times higher than the latter . the hyuc dna fragment was inserted into ppic3 . 5k plasmid to construct the ppic3 . 5k - hyuc recombinant plasmid which was then transduced into pichia pastoris gs115 cells after being linearized by bgl ii digestion
结果表明， sds - page分析在50kd处有一较强的表达带，融合有分子伴侣的重组菌株bl21 ( de3 ) pt221 - hyuh与非融合表达的重组菌株m15 pqe60 - hyuh相比，乙内酰脲水解酶的表达量低一些，但其表达蛋白主要以可溶性形式存在，而m15 pqe60 - hyuh中表达蛋白则主要以包含体形式存在，且前者的酶活性是后者的2倍多。 | | 5. | Multi - copies insertion transformants were screened on g418 plates . the recombinant protein was proved to have biological activity of hydrolyzing n - carbamoylphenylalanine into phenylalanine through enzyme activity assay . the n - carbamoylase activity of recombinant was 2 . 26 and 2 . 15 times higher than that of arthrobacter bt801 and dh5a / puc18 - 169
将hyucdna片段连接到真核表达载体ppic3 . 5k上，经bgl酶切线性化，通过peg法转化导入毕赤酵母gs115感受态细胞，利用g418抗性筛选得到12个插入多拷贝目的基因的转化子。 | | 6. | In this paper , first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv . the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector . after transforming e . coli dh5 a , ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr . presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc . comparing the aquired sequence of 3abc with that of reference strains , the homology is more than 99 percent . the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo . lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene , which happened to form a terminator codon behind 3ab gene , but it contained the complete open reading frame ( orf ) of 3ab gene . positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ) , bacteria were detected by sds - page and western blotting after properly treated . the results showed that the 3ab gene expressed successfully in e . coli and 33 . 5ku fusion protein can be recognized by the positive bovine serum of fmdv . the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
扩增产物连接到pgem - teasy载体中，转化大肠杆菌dh5菌株，筛选氨苄青霉素抗性菌落，提取质粒经酶切鉴定、 pcr分析以及确证性测序证明，所克隆的1500bp左右的片段含有完整的3abc基因，与国外参考序列相比，同源性在99以上。将重组质粒pgem - 3abc和表达载体ptriex - 4neo分别用sal和bgl与xho和bgl消化后，亚克隆3abc基因至原核表达载体ptriex - 4neo中，通过酶切鉴定、 pcr扩增以及序列分析，发现克隆到ptriex - 4neo载体上的片段于3abc基因708bp处出现了17bp的缺失，碰巧在3ab基因后形成一终止密码子，但3ab基因的阅读框架完整，选出含有3ab基因完整阅读框架的阳性克隆，用iptg诱导表达，收集菌液进行sds - page电泳、 westernblotting分析，结果表明， 3ab基因在大肠杆菌中成功表达，其表达产物为分子量33 . 5ku的融合蛋白，并能被口蹄疫病毒阳性血清识别。经薄层扫描分析，表达量占总蛋白量的26以上。 | | 7. | The fragments were ligated directly to the pichia pastrois expression vector ppic9 to got ppic9 - e3and ppic9 - e8 . vectors were amplificated in the e . coli dh5 a and were linearized with bgl ii . the linearized vctors were transformed into host strain gs115 . the recombinated strain was selected though phynotype and pcrthe positive strain was induced with methyl alcohol and was selected by dot - elisa . the recombinated protein was detected with sds - page and western - blot as before
重组菌用甲醇诱导表达，用dot - elisa的方法筛选到表达量较高的菌株。将筛选出的菌株大量的诱导表达，对表达上清处理后，用sds - page和western - blot进行鉴定。同时，用hiprep16 10heparinff肝素亲和柱对表达蛋白进行了初步的纯化。 |
- Similar Words：
- "bgi shenzhen" English translation, "bgijinglvorysculpture" English translation, "bginkgolide b" English translation, "bgj borgafjordur" English translation, "bgkgtjh = traffic is cheacars" English translation, "bgl baglung" English translation, "bgla" English translation, "bglb" English translation, "bgm" English translation, "bgm background music" English translation
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