bgl meaning in English
井眼几何形状测井
Examples
- Bgl bulgaria leva
Bgl保加利亚利瓦 - As heterologous probe and subsequently show to code for desired enzymatic activity . after a serial of subcloning coupled with southern hybridization and enzymatic activity assay , the functional s . griseus atcc14811 cholesterol oxidase gene ( chog ) was localized onto 2 . 3kb ecori - sall fragment
对phz1140和phz1141进行bamh及bgl的酶谱分析及与choa探针的杂交，将胆同醇氧化酶基因初步定位在8 . 8kbbamh和9 . 9kbbgl片段上。 - Based on the restriction endonuclease sites of the reference sequences of a , b , e subgroups published in ncbi , the dna was digested by restriction endonucleases bgiii , bamhi , sspi . the expected results were obtained when digested by bgiii specific to a subgroup or sspi specific to a and e subgroups
结果发现，扩增到的目的带只能用对a亚群特异的bgl限制酶或对a 、 e亚群特异的ssp限制酶切开，而不能用对b亚群特异的bamh限制酶切开。 - Though the expression level of bl21 ( de3 ) / pt221 - hyuh was lower than that of m15 / pqe60 - hyuh , the target protein of bl21 ( de3 ) / pt221 - hyuh was principally in soluble form while the objective protein of m15 / pqe60 - hyuh was principally in insoluble form . both of the products in the two strains showed biological activity , but the former is 2 times higher than the latter . the hyuc dna fragment was inserted into ppic3 . 5k plasmid to construct the ppic3 . 5k - hyuc recombinant plasmid which was then transduced into pichia pastoris gs115 cells after being linearized by bgl ii digestion
结果表明， sds - page分析在50kd处有一较强的表达带，融合有分子伴侣的重组菌株bl21 ( de3 ) pt221 - hyuh与非融合表达的重组菌株m15 pqe60 - hyuh相比，乙内酰脲水解酶的表达量低一些，但其表达蛋白主要以可溶性形式存在，而m15 pqe60 - hyuh中表达蛋白则主要以包含体形式存在，且前者的酶活性是后者的2倍多。 - Multi - copies insertion transformants were screened on g418 plates . the recombinant protein was proved to have biological activity of hydrolyzing n - carbamoylphenylalanine into phenylalanine through enzyme activity assay . the n - carbamoylase activity of recombinant was 2 . 26 and 2 . 15 times higher than that of arthrobacter bt801 and dh5a / puc18 - 169
将hyucdna片段连接到真核表达载体ppic3 . 5k上，经bgl酶切线性化，通过peg法转化导入毕赤酵母gs115感受态细胞，利用g418抗性筛选得到12个插入多拷贝目的基因的转化子。