| 1. | The line corelation between cd62p reserve and in vivo residual life ? an of 22 ? stored platelet were analyzed
采用新鲜富含血小板血浆( fprp )为阴性对照，以凝血酶激活fprp为阳性对照效果良好。 |
| 2. | So called positive contrasts even if be pattern plate with purpose extract , negative contrasts is with empty carrier ( carry la ban ) for pattern plate
所谓的阳性对照就是以目的片段为模板，阴性对照是以空载体（挑蓝斑）为模板。 |
| 3. | Note : “ + ” denotes bacterial growth and “ - ” denotes no bacterial growth . the positive control had bacterial growth and the negative control had no bacterial growth
注： “ + ”表示有菌生长， “ - ”表示无菌生长。阳性对照有细菌生长，阴性对照无菌生长。 |
| 4. | 5 . in this study , we have cloned camv35s and fmv promoter , nos terminater , mark gene nptii , and aim gene pat , epsps and cryia ( b ) genes used as positive collate
5 、克隆了camv35s 、 fmv启动子、 nos终止子、标记基因nptll 、抗除草剂基因epsps 、 pat 、抗虫基因cry1ao ）的阳性质粒作为转基因植物检测的阳性对照。 |
| 5. | Methods 1 ) statistic methods including factorial experiment was carried out to optimize the major conditions for sample management , and the feasible negative and positive control for fcm analysis of cd62p expression were check out
方法1采用浓度梯度法优化gprp浓度条件，采用析因设计优化凝血酶浓度和37孵育时间条件，寻找最佳阴、阳性对照。 |
| 6. | It is efficient for flow cytometric analysis when fresh platelet riched plasma ( fprp ) was set as negative control , thrombin actived fprp and liquid nitrogen treated fprp were set as positive control respectivelv
除了fcm常用的同型对照外，文中还将fpr 、凝血酶激活fprp和液氮处理fprp作为血小板标准阴性、阳性对照，直接应用于fcm分析，且效果良好。 |
| 7. | 5 . prepare the oligonucleotide microarray add 16 prepared probes into 384 - pole plate , meantime , add p2 , low homologic probes and probe buffer in the same concentration as positive control , blank control and negative control respectively . spot microarray as designed by the spotting machine
5 .寡核昔酸微阵列的制备以同等浓度的反义链引物咫作为阳性对照，以探针缓冲液作为空白对照，以无关探针作为阴性对照，与制备好的16条寡核昔酸探针，同时加人384孔板。 |
| 8. | Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s , . aresag " as l ined by saci enzyme . the single fungus , as choose and dibble inocu1ating in we and am plate , the positive fungus was gro ' ing in rm but not in w , and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml . 5 transformers were ampl if ied by pcr , three is same with positive control
选取单个菌落分别点种到删平板和md平板，找出在回d上生长正常， w上生长缓慢或不生长的菌落，即阳性菌落，再以阳性菌落分别涂布zeocine含量500ug加， 1000ug砌l的ypd平板，以高浓度的抗生素筛选高拷贝的酵母工程菌，在含500ug ml高浓度抗生素平板上获得了15个转化子，取其中5个进行pcr扩增，有3个扩增产物与阳性对照相同，说明此酵母细胞中已含有s hbsag融合片段，其中之一命名为p |
| 9. | Methods : the methods of the increase of blood capillary permeability of the abdominal in mice induced by injection of acetic acid and the swelling of the ear in mice caused by crotin were used for anti - inflammatory experiments , aspirin and hydrocortison were respectively used as the positive control ; the methods of abdominal pain in mice induced by acetic acid and woolfe - macdonald were used for analgesic experiments , morphine hydrochloride as the positive control
方法：小鼠随机分组，抗炎实验用醋酸诱发小鼠腹腔毛细血管通透性增高和用巴豆油诱发小鼠耳肿胀，阳性对照分别为阿斯匹林组和氢化可的松组；镇痛实验采用醋酸诱发小鼠腹痛和用热板诱发小鼠足痛，阳性对照为盐酸吗啡组。 |
| 10. | In the third experiment , we first constructed a test model of differentiation from mouse es cells to cardiomyocytes . then series of dilutions of three chemicals were screened on this platform . the combination of all trans retinoic acid and dmso is employed as positive control
实验三首先建立了一个小鼠胚胎干细胞定向分化成心肌细胞的实验平台，然后采用了3种心肌疾病相关的药物：黄芑生脉饮、 1 ， 6二磷酸果糖和hg (代号) ，在此定向分化的实验平台上进行了一系列浓度的药物筛选，实验采用视黄酸和dmso的混合诱导剂作为阳性对照。 |
