| 1. | Overexpression and function analysis of kir2dl4 expressed in nk - 92 cell lines 92细胞上的超表达及其功能分析 |
| 2. | Atmc4 - overexpression in sos2 mutant resulted in enhanced activity of cytochrome oxidase . the transgenic plants showed higher salt tolerance than sos2 而超表达atmca基因sos2突变体植株中,细胞色素氧化酶的活性得到提高,并且转基因植株对盐的耐受性也提高。 |
| 3. | It was also found that the average number of germinated pollen grains on each stigma of ga mutant was less than that of wild type , while in ga overexpression strains the average number was more than that of control ( wild type and empty vector control ) 体内实验中, gpa1突变体柱头上实际萌发的花粉粒数少于野生型,超表达植株则多于野生型和空载体对照; agb1 - 1突变体柱头上实际萌发的花粉粒数与对照比较差异不显著。 |
| 4. | The results represented upon observation of stomatal movement showed that the density of stoma in the epidermal of transgenic tobaccos was much lower than that of wild type and the rate of opening and closing of stoma increased because of strong expression oi atdofl . 7 我们利用rt - pcr的方法,从拟南芥中克隆了与在气孔中表达较多的stdof1基因的同源基因atdof1 . 7 ,构建了该基因的超表达载体,获得了t1代转基因烟草苗。 |
| 5. | The results were as followed : pollen germination and tube growth were decreased in ga mutant , and increased in ga overexpression strains as compared to wild type , while pollen germination and tube growth were not affected in gp mutant 结果表明:体外实验中gpa1突变体花粉的萌发率及花粉管生长速率均低于野生型花粉,而超表达g花粉的萌发率及花粉管生长速率则高于野生型及空载体对照; g突变体agb1 - 1与野生型花粉的萌发率及花粉管生长速率差异不大。 |
| 6. | This paper summarizes the commonly used methods for the research of gene function of filamentous fungi , such as transposon tagging , gene knockout , rna interference , over - expression and yeast hybrid system , and provides a discussion on the advantages and disadvantages of those methods 本文总结了目前丝状真菌基因功能研究中常用的方法,如转座子标签法、基因敲除技术、 rna干扰、超表达及酵母杂交系统等,并对各方法在丝状真菌研究中的优缺点进行了阐述。 |
| 7. | An effort to engineering plants with elevated aba levels and subsequent stress tolerance has been shown that 9 - cis - epoxycarotenoid dioxygenase ( nced ) is a key enzyme in abscisic acid biosynthesis under drought - stressed conditions . plants overexpressing nced showed an improvement in drought tolerance . however , plants maybe have not good growth with too high level of aba in the plant body 目前通过提高aba水平来提高植物的逆境胁迫耐力的研究表明9 ?顺式环氧类胡萝卜素双加氧酶( nced )是干旱胁迫下aba合成中的关键酶,并且超表达nced基因可以提高植株的抗旱性;但是如果植物体内积累过多的aba可能会对植物生长造成不良影响。 |
| 8. | Here we found g proteins also function in leaf , silique development and the yield of pollen microspore . we observed several traits or characters in the offsprings of gpal , agbl null mutation and gpa1 overexpression lines and found that the width of mutants " lamina is larger than that of the wild type , whereas the lamina length , petiole length and rosette diameter is smaller than the wild type , the ga overexpression lines is different from the mutants ; the silique length and the pedicel length is larger in mutants than that of wild type , and slightly smaller in overexpression lines than the control ; the morphometric character in silique tip is different in gpal from agbl mutants ; the yield of pollen microspore is larger in null mutants than wild type whereas smaller in overexpression lines 实验中我们跟踪观察了多代异三聚体g蛋白a亚基超表达转基因植株及a , p亚基缺失突变体的表型特征,发现突变体的叶片宽度大于对应的野生型,叶片长度,叶柄长度及莲座直径小于野生型,而超表达植株的上述某些特征与突变体相反; gp时突变体的长角果长度,花梗柄部长度大于野生型,而超表达ga植株种英则略小于对照; gpal突变体长角果尖端未出现咭乙i突变体的特征: gpal ,口gbl突变体花粉生成量大于野生型,而超表达ga植株的花粉生成量则略小于对照。 |
| 9. | In the experiments discussed in chapter 5 we generated two recombinant viruses based on an acmnpv - and hasnpv - bac - to - bac system , respectively . in such recombinant viruses the busuctl gene under polyhedrin promoter was inserted into polyhedrin gene locus . a preliminary bioassay was conducted 第五章利用杆状病毒bac - to - bac系统构建了含有油桐尺蠖核多角体病毒的类蜗牛毒素基因的重组病毒racbacctl和rhabacctl ,在其相应宿主甜菜夜蛾和棉铃虫的细胞水平和虫体水平进行了超表达实验。 |