| 1. | It was established that grb - ast3 concatemer are more easily expressed in the way of fusion expession
初步认为grb - ast _ 3串联体基因适宜采用融合表达载体表达。 |
| 2. | Construction and expression of recombinant eukaryotic expression plasmid of ubiquitin and epstein - barr virus nuclear antigen 1 fusion gene
病毒核抗原1的融合表达载体的构建及表达 |
| 3. | It was confirmed by restriction enzyme digestion analysis that egfp fusion expression plasmids of scfvs were successfully constructed
的序列正确，经酶切鉴定证实成功地构建了绿色荧光蛋白基因融合表达载体。 |
| 4. | So , we subcloned emt - 1 gene into the prokaryotic fusion expression vector prsetb and generated a 24ku protein
为此将emt l氨基末端基因克隆入融合表达载体prsetb中，转化大肠杆菌bl 21 ，经iptg诱导，表达his6 emt l融合蛋白，表达产物分子量约为24ku 。 |
| 5. | The orf of the hbrp coding a peptide of 233 aa , using the dnasis v2 . 5 demo analysis its structure and pi 3 . screening 1 ) screening using restriction enzyme
Pcr产物和pgex一sx一1融合表达载体分别用bamhi和x五01限制性内切酶双酶切后连接，构建融合表达载体。 |
| 6. | The l - scfv genes were inserted into the eukaryotic fusion protein expression vector pegfp - n3 and transfected transiently into cos - 7 cells to express respectively
将所构建的单链抗体基因克隆入绿色荧光蛋白融合表达载体pegfp - n3 ，瞬时转染cos - 7细胞，分析其表达情况。 |
| 7. | Gst - fusion and native rip from gp & nlaphyuum were expressed in e . coli by inserting the gp609 and rhxjb into the pgex - 2t vector , and dna - 8001 into the pet - 5a vector , respectively
分别将gp609和侧出口b转入pgex一zt融合表达载体，在大肠杆菌dhs 。菌株中实现融合表达。 |
| 8. | Target gene was cloned into the procaryotic fusion expression vector pet28a ( + ) , then subcloned into the eucaryote expression vector pcdna3 . 1 ( + ) after sequence analysis
将halr基因克隆到原核融合表达载体pet28a ( + )上，序列分析后亚克隆到真核表达载体pcdan3 . 1 ( + )上。 |
| 9. | The recombinant fusion protein was highly expressed in e . coli bl21 induced by immol / l iptg in the form of inclusion bodies . the molecular weights of gst - h a1 is 62kd
将构建好的融合表达载体，在1mmol l的iptg诱导下，在大肠杆菌bl21中得到大量表达，经过4h表达量既达到高峰。 |
| 10. | The results indicated that : ( 1 ) marinated in 1 % agno3 for 15min was the optimal sterile condition of seeds . ( 2 ) 2 , 4 - d had great effects on the formation of callus , and the optimal concentration was 0 . 1mg / l
在pbi121vp7表达载体成功构建的基础上，利用质粒pbi121vp7和质粒puc19ctb上相同的ndei和xbai单限制性酶切位点，构建了pbi121ctbvp7融合表达载体。 |
