| 1. | Improvement of adcmv - gfp gene transfection efficiency induced by heavy - ion beam irradiation on murine melanoma cells
离子束辐射对用带有绿色荧光蛋白基因的缺陷性腺病毒 |
| 2. | Production of transgenic embryos expressing green fluorescent protein by nuclear transfer from different types of somatic cells in pig
不同类型的转基因细胞为核供体生产猪的转绿色荧光蛋白基因克隆胚胎 |
| 3. | It was confirmed by restriction enzyme digestion analysis that egfp fusion expression plasmids of scfvs were successfully constructed
的序列正确，经酶切鉴定证实成功地构建了绿色荧光蛋白基因融合表达载体。 |
| 4. | 4 , an intron sequence was also inserted upstream of gfpmut3 and its six reading frame could all be stopped , which could guarantee gfp translation in right reading frame
将蓝色荧光蛋白基因bfp克隆到pet - 11c上，转化bl21 ( de3 )后实现了bfp在大肠杆菌中的诱导表达。 |
| 5. | The symbiosis between mesorhizobium huakuii and astragalus sinicus is a chinese - characteristic symbiotic nitrogen fixation system , while molecular genetic study on its early symbiotic interaction is still at primary stage
本文对新型报告基因?绿色荧光蛋白基因在华癸中生根瘤菌-紫云英共生固氮体系早期结瘤阶段分子遗传学中的应用进行了探索性研究。 |
| 6. | Based on the foundation research of the interaction of virus and host actin , we recombine gfp gene - a reporter gene - with 5c actin gene of drosophila melanogaster , a gfp - actin fusion gene was obtained
本文在总结前人关于病毒与宿主肌动蛋白相互作用的研究的基础上，利用绿色荧光蛋白基因为标记基因，与果蝇肌动蛋白基因5cactin基因融合，构成gfp - actin融合基因。 |
| 7. | 2 . expression of gfp gene in b . thuringiensis strain green fluorescent protein gene is a very useful report gene which could be detected easily . it can be used in the detection of b . thuringiensis environmental release and study of the insecticidal mechanism
绿色荧光蛋白基因在苏云金芽胞杆菌中的表达绿色荧光蛋白基因是一种容易检测的报告基因，可用于苏云金芽胞杆菌制剂施用后的环境监测，杀虫机理等方面的研究。 |
| 8. | In the research of transgenic fish , green fluorescent protein gene was sub - cloned to downstream of carp p - actin gene promoter that was cloned in pucusa by molecular recombination technology . thus pagfp plasmid was constructed successfully . the recombination was determined by digestion of restriction enzyme and sequencing
实验通过分子重组技术，采用定向克隆法将绿色荧光蛋白基因亚克隆到puc118a上鲤鱼-肌动蛋白基因启动子下游，构建成能在真核生物体内表达的表达载体pagfp ，经双酶酶切法序列鉴定后，回收带启动子和目的基因片段。 |
| 9. | Green fluorescent protein has several good characters . under excitation of long uv light or blue light , it emits green fluorescence without requiring any exogenous substrates and cofactors . gfp gene expression can be used to monitor gene expression and protein localization in living cells and organisms . this is a development of revolutionary significance . the dna sequence of this gene can be re - engineered by mutagenesis and the gfp will get improved fluorescent properties . the applications of gfp will be wider and wider
绿色荧光蛋白具有优良的特性，在蓝光或长紫外光的激发下，不需要任何外源底物或内源辅助因子的参入就能发出绿色荧光.绿色荧光蛋白基因的表达可用来监控活细胞或生物体中基因表达和蛋白质的定位.这是一个革命性的进展.而且，对基因dna序列的改造有可能使绿色荧光蛋白的发光特性更加优良，从而其应用范围会更加广泛 |
