Methods for quantitative analysis of immobilized trypsin 备用胰蛋白酶的定量分析法
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The hydrochloric acid in gastric juice is the main cause of the inactivation of pigg 相对而言,胃酸造成的变性要较胰蛋白酶的水解对pigg活性的影响更大。
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2 ) hanks " buffer of trypsin not being added edta could obtain better the growth potential and the subsequent attachment of harvested skin cells t then it being added edta have better digested potent 与添加edta相比,用只含胰蛋白酶的hanks '液处理贴壁细胞,所回收的传代细胞存活能力较好;而添加edta的胰蛋白酶消化液消化能力较强。
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3 ) dispased cold digested single cell is the best to obtain pured epithelial cell line ; it is appropriate to obtain pured fibroblast cell line that repeated attachment and the pured method to their different sensibility to trypsin 对于秦川牛皮肤上皮细胞与成纤维细胞的纯化: dispase冷消化单细胞培养的效果最佳,可获得纯化的上皮细胞系,而根据二者对胰蛋白酶的敏感性不同的分离方法、反复贴壁法适宜于获得纯化的成纤维细胞系。