| 1. | 34 field samples , which were collected from different places of guangxi during 1993 to 2003 , were detected by using developed primary rt - pcr and nested - pcr
结果基础rt - pcr方法检测到其中11份病料为阳性， nested - pcr则检测到23份阳性。 |
| 2. | The transportation of animal epidemic materials for special need in scientific research , teaching and epidemic prevention shall conform to the relevant state provisions
因科研、教学、防疫等特殊需要，运输动物病料的，应当按照国家有关规定运输。 |
| 3. | The new york times reported last week that china failed to send tissue samples of affected pigs to labs outside the country and the lack of transparency about the disease was creating fears of a global pandemic
纽约时报报道上周中国没有给国外的实验室发送染病猪的病料样品，关于该病疫的缺乏透明性行为导致了全球性的恐慌。 |
| 4. | The results of antigen substitution and antigen blocking experiments showed the aci - elisa has highly spectific reactivity with csfv . at last , the aci - elisa was used to test 30 samples , it yielded nearly the same results as pcr
最后用aci - elisa与pcr对30份病料的检测结果比较，表明aci - elisa与pcr检测结果有很高的符合率。 |
| 5. | And wibdv specific rt - pcr also uesed to detected these samples in the years from 1993 to 2000 , there are 8 strains belong to wibdv and 13 belong cibdv . two strains ca n ' t typing for they can be cleaved with both of enzymes sspl and saci .
结果发现1993年至2003年的病料或分离的毒株中，有8株确定为vvibdv ， 13株踊定为cibdv ，另外，有两株不能确定。 |
| 6. | The results demonstrated that the developed rt - pcr based protocol is a specific , sensitive and rapid diagnostic technique for apmv - 1 infections in different poultry species and can also be used to determinate the virulence of the pathogens
应用该方法对源于各种禽类的67份可疑apmv刁感染的病料样品进行检测试验，结果阳性检出率为80石，与常规方法检测的结果本一致。 |
| 7. | Applying the rapid diagnosis technique developed in the laboratory to the suspected tumor samples or dna kept in the laboratory . 32 of 250 samples were identified as avian leukosis viruses ( alvs ) ( amounted to 12 . 8 % of the samples )
应用本实验室建立的肿瘤病快速鉴别诊断技术，对临床送检的苍白消瘦、有肿瘤病变或肝脾肿大的患鸡病料或本实验室保存的dna样品250份进行alv的检测。 |
| 8. | The rna genome of virus used for rt - pcr can be isolated not only from the infected chicken embryonated fluids , but also directly from tissue homogenate of field samples . the detection results of 67 field samples were identical with that of the conventional diagnosis
本方法只需一个rt - pcr反应，整个过程在8小时内完成，既可取感染鸡胚尿囊液检测， 2002年）丫h人《 ‘ wif寸它义：也可从病料直接检测。 |
| 9. | Three ibdv strains , designated as zj2000 , zj991 and zj992 , were isolated from bursa of fabricius of the sick chicken flocks in hangzhou , shenzhou , and ningbo of zhejiang province . the isolates could react with ibdv positive reference serum in the agar immune - diffusion test and caused 30 - 100 % mortality when passaged in chicken embryos . chickens inoculated with these isolates exhibited clinical and pathological signs typical of infectious bursal disease
从浙江省杭州、嵊州，宁波等地暴发疑似传染性法氏囊病的鸡群中采集法氏囊病料(编号分别为zj2000 、 zj991 、 zj992 ) ，分离病毒，经琼脂免疫扩散试验、动物回归试验、鸡胚回归试验和电镜观察等证实所分离的3株野毒是传染性法氏囊病病毒。 |
| 10. | In this experiment , three dead sheep from spa were been as materials to make histopathological and ultrastructural observation . the results showed the primary lesion was the resultant transformation of type ii epithelial alveolar cells . tumors were composed mostly of single layers of cuboidal epithelial cells arranged in an acinar pattern , but all rumors had areas where the tumor cells piled up , forming papillary projections into the alveolar spaces
本试验以某羊场病死羊肺脏作为病料，制成病理组织切片，通过病理组织学检查，结果表明：肺脏主要病变是肺泡细胞变为立方形或低柱状细胞，这些细胞沿肺泡壁排列，突入肺泡腔内形成乳头状腺瘤结构；在气管，支气管腔内有灰白色泡沫状液体，严重时使肺泡变为腺管状等。 |
