There exist successive deletions in the sequence of the genomic dna of kale and kohlrabi , compared with that of brussel sprouts 相对抱子甘蓝基因组dna而言，羽衣甘蓝和球茎甘蓝在序列中发生了多处大量碱基的缺失。
2.
Primers were designed according to the sequences of atcal , bocal , bobcal and boical gene , and pcr amplifications were performed with the genomic dna of brussel sprouts , kale and kohlrabi respectively , pcr fragments of about 1 . 6kb were obtained and designated correspondingly as bogcal5 " , boacal5 " and boccal5 " 根据拟南芥atcal 、结球甘蓝bocal 、花椰菜bobcal和青花菜boical基因设计引物，对芸薹属植物抱子甘蓝、羽衣甘蓝和球茎甘蓝的基因组dna进行pcr扩增，均得到一段约1 . 6kb大小的pcr产物，分别命名为bogcal5 ’ 、 boacal5 ’和boccal5 ’ 。
3.
However , the discrepancy has no remarkable affect on the relationship of them . 2 . sequence alignments between the three fragments of according cdna to find that boacal and boccal share 97 . 9 % identical percentage while the identity percentage between bogcal and boacal , boccal are 98 . 9 % , 98 . 2 % , respectively 2 、对分离的三段序列对应的cdna序列之间进行了同源性比较，结果羽衣甘蓝和球茎甘蓝之间为97 . 9 ，抱子甘蓝同羽衣甘蓝和球茎甘蓝之间分别为98 . 9和98 . 2 。
4.
Reclamation , purification and linkage of them respectively , then sequencing and analyses of according gene structure were made , results show that the complete sequence length of corresponding pcr product from brussel sprouts , kohlrabi and kale are 1665bp , 1650bp and 1650bp , containing the first two exons and introns and 22bp of the third exon 对各pcr产物分别进行回收、纯化、载体连接和序列测定及基因结构分析等，结果表明，该片段在抱子甘蓝、羽衣甘蓝和球茎甘蓝三种作物中的全长分别为1665bp 、 1650bp和1650bp ，包括相应基因的前两个外显子和内含子以及第三个外显子的22bp序列。
