Chinese translation for "清经"

clear menstruation-heat
kiyotsune

Example Sentences:

	1.	The way of the hidden books of high clarity in the six dynasties 六朝上清经的隐书之道
	2.	On the origins of the names of daoist deities and the visualization practice of supreme clarity school in the eastern jin dynasty 兼论东晋上清经派存思修炼法门
	3.	The secretive expression of scfv fusion proteins were confirmed by sds - page and western blot analysis in cell split products and condensed supernatant 建系细胞裂解产物和浓缩的培养上清经sds page及westernblot分析，证实了scfv融合蛋白的分泌性表达。
	4.	Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid , and induced with methanol to secrete interesting peptide . the supernate of pichia pastoris culture was analysed by sds - page and western blotting . a reactive band , which the apparent molecular weight is 36kd , can be detected with sheep anti - hcmv polyclonal antibodies 重组质粒转化巴氏毕赤酵母， g418筛选出多拷贝插入的单克隆，甲醇诱导多拷贝插入的单克隆酵母细胞分泌目的蛋白，培养液上清经sds - page电泳分析，在蛋白质印迹中检测到培养液上清有一表观分子量为36kd ，能与羊抗hcmv多克隆抗体发生发应的条带。
	5.	The hwtx - i gene was chemically synthesized according to its known cdna sequence , the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor , the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis , then it was transformed into host strain gs115 , a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations , the multicopy transformant was named gh1 . gh1 was cultivated in flasks . after 6 days of induction by 0 . 5 % methanol , the supernatant was checked by 16 . 5 % tricine - sds page , which showed there was a band in the position of 3 . 5 - 6 . 1kd , then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column , after reverse phase hplc of ci8 and vacuum drying , the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page , maldi - tof mass spectrometry , amino acid composition analysis , the n - terminal amino acid sequence and its biological activity , the final field of the purified rhwtx - i was about 80mg / l , accounting for 23 . 6 % of it total secretory proteins 将带有hwtx -基因的ppic9k经blgii线性化后，转化酵母宿主菌gs115原生质体后经筛选阳性克隆并经表型鉴定为his ~ + mut ~ s酵母菌，进一步用遗传毒素g418筛选多拷贝的转化菌株，命名为gh1 ；将gh1甲醇酵母菌用0 . 5的甲醇诱导表达，发酵上清经90饱和度的( nh _ 4 ) _ 2so _ 4沉淀， yw - 3 ( mwc03000 )的超滤膜超滤，再经cm阳离子交换， c _ ( 18 )反相hplc纯化得到分子量为4kd左右的组分，其中4289 . 05的组分经质谱鉴定，氨基酸组成分析和序列测定为正确的表达产物，生物学活性表明其活性为天然毒素活性70 % ，表达量为80mg / l 。

Similar Words：

"清津机场" Chinese translation, "清进实业公司" Chinese translation, "清近" Chinese translation, "清劲……风，风势间中疾劲" Chinese translation, "清劲风" Chinese translation, "清经热" Chinese translation, "清经散" Chinese translation, "清井" Chinese translation, "清井捞筒" Chinese translation, "清静" Chinese translation