Remember , in order to register a cultivar you need to take it through three or more generations . your new cultivar should reproduce true at least 95 % of the time 确保你的新品种历经三代或是更多子代后， 95 %以上的机率要跟母株一样，确定植株的稳定性。
2.
Plant population varies from 1 , 400 to 1 , 600 plants per hectare and the plantation is kept in the field for several ratoons using suckers coming from the mother plant 种植密度保持在1400株/公顷到1600株/公顷，种植园田地上留下母株的根出条所形成的截根苗。
3.
You have reproduced the plant vegetatively ( leaf cutting or , in the case of a chimera , sucker propagation ) through at least three generations , and the offspring have reproduced true to the original plant 至少繁殖三代以上(叶子,侧芽或是砍头繁殖)且繁殖的小孩的花朵颜色型态都要跟母株完全一样。
4.
An example is mangroves , in which the seeds germinate into good - sized seedlings before they are released from the plant , enabling them to plunge far enough into the mud to establish themselves 例如红树林的种子可以在脱离母株前发育成相当大体积的树苗，这使幼苗可以栽入足够远的淤泥中进行自身的巩固发展。
5.
All of the genetic factors , maternal environment , storage conditions , collecting methods and the degree of maturity , moisture content , size , shape and colour of seeds can affect hardseed percentage 遗传因素、母株环境、贮藏条件、采收方法、种子本身的成熟度、含水量、大小、形状及颜色都能影响种子硬实率。
6.
In this paper , the progress of isolated microspore culture was reviewed : the major factors affecting regeneration ; the methods of reduplication ; the major factors affecting the formation of embryo , for example , experimental material , culture medium , raise condition , a phase of microspore growth 通过对影响大白菜游离小孢子胚胎发生的主要因素材料基因型、供体母株生长状态、小孢子发育时期、培养方法、培养条件和影响植株再生频率的因素以及小孢子植株的加倍方法等进行了综述。
7.
Therefore , the above two areas were chosen to select five maternal plants , then branches collected from the five maternal plants were used to carry out cutting seedling test , results showed as the follows : when one - year - age branches without treating were applied in cutting seedling test , the average survival rate reached to 88 % 从以上2个片区选取了5个优良母株插条进行?插试验，结果表明： 1年生幼年枝未经任何处理?插平均成活率达到88 % ；成年枝?插，经过吲哚乙酸浸泡处理比无处理平均成活率显著提高，达到68 % ，而无处理平均成活率仅有29 . 2 % 。
8.
2 - e4 - a and 82 - 6 are hybridized during their log growing time , and the hybrid - hybridomas are cloned for 3 times and produce 6 hybrid - hybridoma cells . the chromatosome of hybrid - hybridoma 3 - hu and hybridoma 2 - e4 - a and s2 - b are counted , and the antibody of ascites fluid or culture supernatant of 3 - hn is prepared . the positive clones are detected by three methods at the same time : rbc agglutination for monospecific anti - human rbc type a antibody , indirect elisa for anti - p24 antibody , and rbc solid - phase adherence for bispecific antibody 选其中一株3 - h _ ( 11 )做杂交-杂交瘤细胞染色体计数，同时计数两母株2 - e _ 4 - a和s _ 2 - b的染色体数：制备腹水型和上清型抗体，用三种方法同时检测其中的双特异性抗体、单特异性抗人红细胞抗体和抗p24抗体，即红细胞固相吸附法测双特异中文摘要性抗体，红细胞凝集试验测单特异性抗人a型红细胞抗体，间接elisa法测抗p24抗体；用腹水型抗体做耐热性及耐冻融实验。
