| 1. | The analyses showed that rapd was able to differentiate mainly at the species level , while eric is effective at the strain level
以黑木耳菌株为探针进行southem杂交，该探针与黑木耳和琥珀木耳均有杂交带，而毛木耳没有。 |
| 2. | Hybridization bands were detected by southern blot analysis using lea3 gene probe labeled by digoxigenin the result showed that foreign lea3 gene integrated into strawberry genome
2 。对点杂交为阳性的植株进行southernblot分析，转化植株检测到了杂交带，除预期的1 |
| 3. | 27kd protein bands were detected in transgenic plants by western blot analysis , and the results showed that the foreign lea3 gene could express in transgenic strawberry plants
Westernblot分析，在转化植株中检测到了27kd杂交带，表明在转化草莓体内有外源基因翻译产物的积累。 |
| 4. | Sds - page showed efficient expression and secretion of the rhfl . the highest yield ( 108 mg / l ) was obtained when expression was induced with 0 . 5 % methanol for 96 hours
Western杂交实验出现了可被抗体特异性识别的杂交带，证实此条带即为重组毕赤氏酵母km7lppicgkil分泌表达的重组fl蛋白。 |
| 5. | Except for 1 . 0 kb hybridization band , other different hybridization bands were also detected which demonstrated that lea3 gene integrated into strawberry genome in different loci or copies
Okb杂交带外，还有其他片段人小不等的杂交带。不同的株系形成不同的杂交谱，表明这些转化植株来自不同的转化细胞。 |
| 6. | The genomic dna extracted from three rubber tree strains ( pr107 , rrim600 , gt1 ) was digested with hinckr . the southern blot was made with the same probe as above . the result showed that all the three strains turned up only one brand ( - - 4 . 8kb ) . the three strains had the same inherent background
用橡胶树pr107 、 rrim600 、 gti三个品系的基因组洲a分别经hinan酶切后，进行了southern杂交。结果表明，三个无性系都只出现一条杂交带，位置一’致（ 4 |
| 7. | By using these antibodies , which were raised to immunoreact with total proteins of purified mitochondria from different organs of mung bean seedlings , we find that there were two hybridizable aox bands in mitochondria in mung bean seedlings . their molecular weight was about 35kd and 38kd respectively
将此抗体与来自绿豆幼苗不同器官线粒体的总蛋白分别进行western杂交，可观察到绿豆幼苗线粒体中具有两条清晰的aox杂交带，它们的分子量分别为35kd和38kd 。 |
| 8. | I and hindlll respectively . after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane , the southern blot was carried out using the cdna of rubber tree etrl as probe . the result of the southern blot showed that a hybridization band ( - 3 . 0kb ) turned up from the ecor i digested product and another band ( - 4 . 8kb ) turned up from the hindi ]
从橡胶树pr107品系的嫩叶提取基因组dna ，用限制性内切酶ecor 、 hinofll分别酶切，琼脂糖凝胶电泳分离并转膜后，用克隆的橡胶树etri基因的cdna片段作探针进行southern杂交分析，结果表明， ecor酶切在约3 okb处有一条杂交带出现， hi 。 |
