| 1. | Detection and bio - assay of insecticidal crystal protein bmb20 - 4 from bacillus thuringiesis
4的杀虫晶体蛋白检测及毒力测定 |
| 2. | Analysis of insecticidal crystal protein and its cry - type genes of bacillus thuringiensis isolates from china
分离株杀虫晶体蛋白及基因分析 |
| 3. | These insecticidal proteins are commonly known as insecticidal crystal proteins ( icp ) or delta endotoxin
这些杀虫蛋白通称为杀虫晶体蛋白（ icp ）或-蛋白。 |
| 4. | Two main parts were included in the present thesis . part i . cloning and expression cryigenes of bacillus thuringiensis in different host strains
一、苏云金芽胞杆菌杀虫晶体蛋白基因在不同受体菌中的表达1 |
| 5. | 3 . construction of fusion genes with insecticidal protein gene and gfp the fusion genes was constructed by pcr . the pesticidal crystal protein gene crylac10 and gfp were chosen to construct the fusion genes
建杀虫晶体基因与gfp基因的融合基因pcr扩增全长gfp片段融合于去掉终止结构的杀虫晶体蛋白基因的c -端，构建融合基因。 |
| 6. | The results of sequencing and structural analysis showed that the gene , named crylaa14 by international nomenclature committee of bt 5 - endotoxin , was 3552 bp and the open reading frame encoded a 133 . 7 kda protein with 1183 amino acids
序列测定结果表明该基因编码区为3552bps ，编码1183个氨基酸，分子量为133 . 7kda ，等电点为pi4 . 755 。该基因序列已在genbank注册， accessionnumber为ay197341 ，并被国际bt杀虫晶体蛋白基因命名委员会正式命名为cry1aa14 。 |
| 7. | The research results are summarized as following : 1 . the relationship between gene types and the toxicity against s . exigua in order to construct satisfactory genetically engineered strain , it is first necessary to understand the relationship between gene types and the toxicity . nine strains containing crylc were chosen for study for this purpose
研究结果总结如下： 1菌株对甜菜夜蛾的毒力与基因类型的关系pcr扩增检测了九个苏云金芽胞杆菌菌株所含杀虫晶体蛋白( insecticidalcrystalprotein , icp )基因，根据扩增结果将它们分为5种基因类型。 |
| 8. | The recombinant plasmids pbmb121l , pbmb9821l and pbmb986l were constructed after transferring bacillus thuringiensis icps gene crylaal , crylaclo and crylca from their original plasmid vectors to different plasmid vector . the relevant recombinant strains were obtained after introducing the 3 recombinant plasmids into bacillus thuringiensis plasmid - free mutant strain 8mb 171 by electroporation . the transformation and expression properties of 8mb 171 were studied
通过将苏云金芽胞杆菌杀虫晶体蛋白基因cry1aal 、 cry1ac10和cry1ca转移至不同质粒载体上，分别构建了重组质粒pbmb121l 、 pbmb9821l和pbmb986l ，并分别导入无质粒突变株bmb171 ，筛选得到携带相应icps基因的重组菌株。 |
| 9. | 4 . effect of resolution vector on the expression of pesticidal crystal protein genes this work successfully introduced pesticidal crystal protein genes into crystal negative strain bmb171 by using resolution shuttle vectors . after resolution , the expression of cry1ac and cry 1c genes have no obvious change , but the expression of cry3a genes has great increase in the same condition
解离反应对杀虫晶体蛋白基因表达的影响成功地利用解离载体将crylac10 ， crylc和cry3a基因导入苏云金芽胞杆菌无晶体突变株， crylac和crylc基因解离前后的表达量和杀虫毒力未见明显变化， cry3a基因在相同条件下则表达量有所提高，至于为何只对基因cry3a有作用尚不清楚，国内外也未见有人作相关报道。 |
| 10. | Microcalorinetric study on b . thuringiensis by using an lkb - 2277 bioacitivity monitor , the thermogenic curves of different b thuringiensis strains ybt - 833 and ybt - 833 - 2 - i , have been determined . the metabolism heat output revealed the heat output was correlated to the yield of the protein , the higher yield protein , the less heat output . a microcalorimetric technique based on the bacterial heat - output was explored to evaluate the effect of various promoters and different plasmid original replicons on the expression of gfp
不同苏云金芽胞杆菌基因工程菌的微量热变化利用生物活性检测器lkb - 2277研究杀虫晶体蛋白含量不同的两株菌ybt - 833 、 ybt - 833 - 2 - 1的热动力学变化，发现菌体合成杀虫晶体蛋白的过程是一个耗能的过程，杀虫晶体蛋白产量高的菌株向外释放的代谢热少，反之亦然。 |
