| 1. | The main results are the following : 1 . the hplap without signal peptide was expressed in p . partoris smd1 168 by the ppicz - a a plasmid . 2
在毕赤酵母几psrtorjssmdi168e株中用表达载体ppiczqa成功表达了去掉信号肽的有活性的碱性磷酸酯酶hplap 。 |
| 2. | When bacteria enter the detector , their normal metabolism cleaves an inactive nutrient into an active one , allowing the detector ' s spores to germinate
当细菌一进入侦测器后，细菌的正常代谢反应就会将一种不活化的营养物质，裂解成有活性的东西，促使侦测器内的孢子开始生长。 |
| 3. | After yellow aspergillus toxin enters the person ' s liver , be released to issue the part that has active by splitting decomposition , action at liver cell , have the effect that cause cancer
黄曲霉毒素进入人的肝脏后被裂解释放出具有活性的成分，作用于肝细胞，具有致癌作用。 |
| 4. | In the stomach , this acid functions to kill bacteria in foods , to soften foods and to convert the inactive enzyme pepsinate into its active from pepsin , to begin the digestion of protein
在胃里，这种酸的作用是杀司食品的细菌，使食物软化，把钝化的胃蛋白酶原转化为有活性的能消化蛋白质的胃蛋白酶。 |
| 5. | In uncultivated compounds database , there have been more than 25 , 000 new - found but not deeply researched compounds , which are valuable resource of leads for drug and pesticide design
收集了最新报道的未被广泛深入研究过的新化合物25 , 000个，主要是有活性的天然产物。这些化合物是新医药创制的重要资源。 |
| 6. | Then the total 25 engineered strains were cultured and induced in the respective conditions . the results show that the target products expressed in 3 strains of them are partly soluble and active , whils
11bl21 ( 0e3 )表达的包涵体蛋白进行了变性、复性研究，部分获得有活性的d一海因酶，酶活力约为0 . 90u / mgo |
| 7. | In this thesis , the hplap gene was cloned into the ppiczaa plasmid and induced expressed in the protease - deficient strain p . pastoris smd1 168 . the clone that could secrete hplap with enzyme activity was selected
本研究将hplap基冈兑隆到spiczoa质粒中并在毕赤酵母尸pastorjs蛋白酶缺陷菌株smdi168中诱导表达，获得了有活性的分泌型hplap 。 |
| 8. | However , the mechanism of action of angiostatin on endothelial cells is less clear . further structural and functional studies are hampered by a lack of soluble , correctly folded , biologically active protein in sufficient auantity
然而， angiostatin对内皮细胞的作用机制并不十分清楚，其结构和功能的研究往往受无法获得足够量的，可溶的，能正确折叠的，有活性的蛋白的限制。 |
| 9. | Addition of iptg to growing culture of the lysogen induces t7 rna polymerase , which in turn transcribe the target dna in the plasmid . in the presence of glucose and appropriate conditions such as temperature and concerntration of iptg , a 52kd protein with tryptopanase activity was expressed
摸索发酵条件，如改变培养温度和iptg浓度等，发现在30培养条件下， 0 . 2mmiptg诱导时，发酵液中的吲哚含量最高，表明低浓度的诱导剂或低温诱导有利于表达出有活性的色氨酸酶。 |
| 10. | Induced either by iptg or l - tryptophan in the absence of easily metabolized carbon such as glucose the strain bl21 ( de3 ) / pet28c - tnaa can express tryptopanase . the fermentation conditions were optimized respectively . in the presence of glucose and iptg as induce agent , the concentration is the crucial factor for expression of active tryptophanase . if the iptg concentration is less than 0 . 2mm , the optimum temperature is 37 lower temperature is necessary to obtain active tryptophanase in the case of higher concentration of iptg
用iptg诱导表达实验结果表明：利用t7启动子在胞内能表达出有活性的酶； iptg与温度的合适组合可以得到较高活性的色氨酸酶，在0 . 2mm以下浓度的iptg诱导时， 37是最适温度，而用较高浓度的iptg诱导，低温是表达有活性的色氨酸酶的必要条件，而且诱导时间要短。 |
