| 1. | Inspection method on newcastle disease virus in poultry for export
出口家禽新城疫病毒检验方法 |
| 2. | The investigation on the molecular epizootiology of newcastle disease virus
中国部分地区新城疫病毒的分子流行病学研究 |
| 3. | In part two , an amperometric immunosensor system for newcastle disease antibody ( nd . ab ) assay has been developed
第二部分工作是研制了一种电流型免疫传感器测定新城疫病毒抗体( nd |
| 4. | The causative agent of the disease , newcastle disease virus ( ndv ) , is a member of the paramyxoviridae whose genome is a non - segmented single - strand negative - sense rna
该病的病原新城疫病毒( newcastlediseasevirus , ndv )属副粘病毒科，为有囊膜的负链rna病毒。 |
| 5. | A dot - enzyme - linked immnosorbent assay ( dot - elisa ) for the detection of antidodies against newcastle diesease virus ( ndv ) was established by using purified ndv and self - made enzyme - labeled anti - chicken igg , the mehod was also evaluated through it ' s application
本研究用提纯的新城疫病毒和自制的酶标抗体建立了检测鸡新城疫抗体的dot - elisa方法。试验中用f _ ( 48 ) e _ 9株和lasota株提纯抗原并对两者进行了比较。 |
| 6. | In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed , hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3
应用反转录-聚合酶链反应( rt - pcr )获得两株新城疫病毒( ndv )血凝素-神经氨酸酶( hn )基因cdna ，然后定向插入真核表达质粒pcdna3中，构建了含hn基因的重组质粒。 |
| 7. | Newcastle disease virus ( ndv ) is grouped under the family of paramyxoviridae , it causes serious infectious disease and incurred great losses to poultry industry . f and hn protein were main glycoproteins , which affect the virulence of the virus
新城疫病毒( newcastlediseasevirus ， ndv )属于副粘病毒科( paramyxoviridae )副粘病毒亚科( paramyxovirinae )腮腺炎病毒属( rubulavirus )的成员，其主要结构蛋白融合蛋白( f )和血凝素-神经氨酸酶( hn )与其致病性密切相关。 |
| 8. | The specificity of the nucleic acid probe was very strict . lt reacted positively with iltv dna only and it react negatively with the nuleic acid of ndv , bv and ibdv . the sensitivity of this kind of probe is very high . ilt could even detect 20pg ' s iltv dna
结果表明：该种核酸探针具有高度的特异性，它仅与iltv的dna呈现阳性反应，而与新城疫病毒、传染性法氏囊病病毒和传染性支气管炎病毒的核酸等均呈阴性反应。该种探针具有高度的敏感性，能够检测到20pg的iltv的dna 。 |
| 9. | All the result showed that ndv f48e9 strain has its own speciality compared with other five ndv strains , and there were many difference between velogenic strains and lentogenic strains . so the infectious cdna of rnesogenic strains and lentogenic strain was far from enough to understand the replication , pathogenicity of ndv and the interaction between ndv and host cells , and the infectious cdna of velogenic strains ( eg . . f48e9 ) was required to explain the relationships between structure and function
本研究成功地获得了ndvf48e9 t因组的核昔酸序列，并构建了表达ndvf48e9基因组cdna的低拷贝表达载体休f48e9 ，为构建新城疫病毒强毒株f48e9株的感染性cdna奠定了物质基础，进一步研究ndv的生物学特性、结构与功能的关系；进一步探讨影响ndv毒力的因素、以及研制新型疫苗载体提供了可靠保证。 |
| 10. | Newcastle disease is a highly contagious and economically most important disease of poultry . the disease also inflicts many other avian species . the disease varies in degree of severity ranging from mild , inapparent infecion to severe disease . based on the severity of the disease in chickens , the causative agent of the disease , newcastle disease virus ( ndv ) , is divided into three pathotypes : ( 1 ) the lentogenic strains , which cause mild inapparent infection ; ( 2 ) the mesogenic strains , which cause mild respiratory symptoms with low mortality ; ( 3 ) the velogenic strain , which are highly virulent and cause high mortality
新城疫( newcastledisease ， nd )是由新城疫病毒( newcastlediseasevirus ， ndv )引起的侵害禽类的急性高度接触性传染病，是家禽传染性疾病中危害最严害的疫病之一，由于对世界养禽业危害极大，故被国际兽疫局确定为类传染病。在传染性疾病的预防中，疫苗是减少易感人群而被最广泛采用和最有效的途径。 |
