| 1. | Research of multiple locus gene targeting in vivo of siniperca
多位点基因打靶技术的鳜鱼体内实验研究 |
| 2. | The development of gene targeting and animal cloning thechnology makes the site - specific integration of foreign gene become possible
随着基因打靶技术和动物克隆技术技术的发展，外源基因在高等哺乳动物基因组中的定点整合成为了可能。 |
| 3. | The moss physcomitrella patens ( funariales bryophyta ) is being developed as a model system for plant biological studies . its gene targeting efficiency can be compared with that observed in saccharomyces cerevisiae
小立碗藓是最近被开发出来的用于研究基因打靶的模式植物，其基因打靶的效率可以与酵母相比。 |
| 4. | All these fragments were cloned into pmd - 18t vector for the enzyme and sequencing analys is . enzyme cutting analysis indicated the enzyme sites suport to establish a targeting vector
把它们分别克隆到pmd - 18t载体上，经酶切分析、部分序列测定和同源分析确认克隆得到了所需的基因片段，可以用于基因打靶载体构建。 |
| 5. | But gene targeting was mainly applied to such field as bacterium , yeast and some epiphyte at present , seldom used in higher plants because of the lower frequency of homologous recombination
但基因打靶技术目前主要应用于细菌，酵母和一些丝状真菌，在高等植物中应用极少。原因是高等真核生物中同源重组的发生频率极低。 |
| 6. | At present , there are several approaches to targeted gene disruption ( or gene targeting , as it ' s usually called ) that can be customised to attack anywhere in the genome , but they ' re all highly error - prone , disrupting other locations a great deal
目前，有几种方法可用于打靶的基因破坏（或称基因打靶，实际名称也是这样） ，它可以定制，用来攻击基因组的任何地方，但它们全都容易出错，在非常多的地方破坏其他位点。 |
| 7. | Gene targeting by homologous recombination can realized targeted integration . at the same time we can knock - out some gene to know its genetic function and create specific mutation to study some kinds of metabolizing mechanism
第一部分：立碗藓原生质体分离及培养的研究基因打靶技术通过利用同源重组可以在一定程度上实现外源基因的定向整合；同时还可通过对某基因的剔除研究基因的功能或通过创造定点突变来研究某种代谢机理。 |
| 8. | 4 . engineering dhqase ( arod ) - deficient e . coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e . coli chromosome . the mutant 31bk was engineered , in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e . coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system . the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene , so it improved carbon flow into the quinic acid biosynthesis direction
构建宿主菌基因精确定位突变株31bk ( arod : : arobkan ~ r )为了改变代谢途径脱氢奎尼酸( dhq )分支点上的代谢流量，使之充分流向目的产物奎尼酸合成方向，利用基因打靶技术构建了31884宿主菌arod基因精确定位插入突变体，使dhq脱水酶( dhqase )失活，阻断了碳代谢流流向芳香氨基酸生成的方向，同时用同源重组的方法将arob基因定位整合入染色体上，解除了限速酶对碳代谢流通过共同途径到达dhq的阻遏影响，并减轻代谢负担。 |
| 9. | Then some necessary elements such as gfp gene , neo gene were inserted between the loxp site and the loxs 11 site and obtained a new gene targeting vector pioxgfp . on the other hand , the chicken v - ifn cdna was cloned into the vector plox between the loxp site and the lox511 site and got another gene targeting vector ploxifn
合成loxp和lox511位点，将其同方向地克隆到载体pegfp - 1的多克隆位点之内，构建成含有lox位点的通用载体? ? plox ，然后将报告基因gfp及neo基因片段克隆到载体plox上的loxp和lox511序列之间，构建成一基因打靶载体? ploxgfp 。 |
