These results suggested that axud1 protein functioned in a different way in the expression of cell cycle and apoptosis related proteins induced by tgf - 1 in tumor cells . 5 . axudl cdna fragment was cloned into procaryotic expression vector pqe30 and t 5 . axudlcdna片段成功地克隆入原核表达载体pqe30 ,并用iptg诱导出axudi融合蛋白的表达,这为进一步纯化axudi蛋白,研究axudi蛋白的免疫组织化学定位和结构功能奠定了基础。