| 1. | Gene promoter on its 5 flanking sequence 基因5末端侧翼序列启动子的分析 |
| 2. | Gene promoter on its 5 flanking sequence analysis of the mouse 基因5末端侧翼序列启动子的分析 |
| 3. | Cloning and analyzing the 5 ' region of follicle stimulating hormone receptor gene on buffalo 端侧翼序列的克隆与分析 |
| 4. | Floral aberrance of cbf1 transgenic tobacco and analysis of its flanking sequences 1基因烟草植株的花器官变异及其插入区侧翼序列的分析 |
| 5. | Dna sequences flanking tn5 - 1063 of 042bmr5 were amplified using inverse pcr , it was found that tn5 - 1063 was inserted into noeb gene 对042bmr5突变株的基因组进行反向pcr ,扩增位于tn5 - 1063两端的侧翼序列。 |
| 6. | The beth gene was predicted to encode a 55 . 2 kda protein ( 504 amino acid residues ) with 12 transmembranes regions 通过反向pcr扩增获得beth基因片段的侧翼序列。通过blast比较,证实获得beth基因的全部编码序列。 beth属于bcct家族。 |
| 7. | Sequences flanking tn5 in the mutants were cloned by self - ligation . because each transposon contains an origin of replication functioned in e . coli , but not in rhizobium 使用质粒自连法克隆了042bm - x1和042bm - x2中tn5插入位点的侧翼序列。 |
| 8. | The transient expression of lacz driven by crtw 306bp s ' - flanking sequence and crtz 302bp s ' - flanking sequence shows that these two sequences habour transcription regulatory sequences 以lacz为报告基因的瞬间表达实验结果表明,长度分别为306bp和302bp的crtw和crtz5 '上游侧翼序列具有很强的启动转录活性,提示两段序列包含了启动子的结构。 |
| 9. | The sequences flanking tn5 in magnetosome deleted mutant nm4 was cloned by anchored pcr , and was used as tag to extend the 5 ' and 3 ' terminal sequences of the target dna fragment by anchored pcr constinuously 以tn5为标签,用锚定pcr法克隆出突变株nm4的tn5侧翼序列。并以此侧翼序列为标签继续用锚定pcr法向两边延伸,克隆出一个5045bp的dna片段。 |
| 10. | Sequences flanking tn5 in nm21 was cloned by anchored pcr . a 3073bp fragment which contains three putative open reading frames ( orf ) and shows strong homology with the magnetospirillum magnetotacticum ms - 1 was obtained , the tn5 inserted in the orf1 用锚定pcr法从nm21中克隆出tn5侧翼序列,得到一个大小为3073bp的dna片段,此片段由3个orf组成,与m . magnetotacticumms - 1的对应片段具有89的同源性。 |