Switch on the vacuum to draw the solution through the column ( the plasmid dna binds to the column material ) 开启真空装置使液体通过管柱(质体dna会与管柱物质结合) 。
2.
Filtration separates solid from a liquid by forcing the liquid through a solid support or filter medium . this is a straight forward procedure for well defined crystals 过滤是在某一支撑物上放过滤介质,注入含固体颗粒的溶液,使液体通过,固体颗粒留下,是固液分离的常用方法之一。