| 1. | The stem sections were better receptors for torenia fournier transformation
在本实验中最高转化率为24 . 1 。 |
| 2. | Putatively transformed shoots all attained from torenia fournieri lind
分别得到了在选择培养基上生长的抗性植株。 |
| 3. | The explants with roots have the highest frequency of torenia calli induction
不同的外植体中，以带有根系的材料的诱导率高。 |
| 4. | The effects of varying conditions on torenia transformation were investigated
不同菌株对蓝猪耳的遗传转化能力不同， r1000和a4明显高于r1205和r1601 。 |
| 5. | And portulaca grandiflora hook . . the ability of calli formation of different torenia explants is distinct
对于蓝猪耳的愈伤组织诱导而言，不同外植体类型之间存在很大的区别。 |
| 6. | Pre - cultured 2 days and co - cultured 6 days can also enhance the efficienty of transformation of torenia fournieri lind
预培养2d后共培养6d ，可以明显增加gus基因的瞬时表达和不定芽的形成。 |
| 7. | Ph value increase from 4 . 5 to 6 . 5 strongly stimulated hairy root formation and higher ph value ( above 7 . 5 ) inhibited it . under the optimized condition , the transformation frequency of torenia approached 90 %
预培养和共培养培养基ph从4 . 5提高到6 . 5时，毛状根诱导率显著提高，但是当提高到7 . 5时，诱根率却显著下降。 |
| 8. | In the experiment , we have explored optimal conditions for the transformation of torenia fournier , and transformated it with antisense acc synthase gene to get transformated torenia fournier plants with flower longevity extension and more tolerance to abiotic stress
本研究探索和完善了蓝猪耳遗传转化条件，并将反义acc合酶基因转化蓝猪耳，以期获得延长花寿命，和有较高抗性的转基因蓝猪耳植株。 |
| 9. | The purpose of this study is to provide theoretical and technical support for torenia transgenic hairy root and other molecular researches used by torenia . the comparation and identification of agrobacterium rhizogenes strains were investigated using 3 - ketolactose test , the differential acid production assay and the motility assay
采用3 -酮乳糖产物法、差异酸生成实验和游动性实验鉴定6种发根农杆菌供试菌株a4 、 r1205 、 r1000 、 r1601 、 r1022和15834 。 |
| 10. | In this research , we constructed the expression vectors , which contain exogenous gene chalcone systhase ( chs ) antisense gene of gerbera hybrida and transformed it into agrobacterium lba4404 through freezing - melting . an efficient regeneration system and transformation system have been estadblished on leaf discs of torenia fournieri lind
以蓝猪耳为主要研究对象，建立了高效的蓝猪耳离体再生体系，并以蓝猪耳叶片为转化材料，用带有目的基因的农杆菌感染建立了蓝猪耳的遗传转化体系。 |
