| 1. | Arbitrary waveform composer software for window 95 sfg - s1 option
任意波形编辑软体- for windows |
| 2. | Cdp - 12w intelligent weighing filling machine sp2 - 83s two - in - one conveying belt sfg - 2 automatic powder - adder
Sp2 - 83s二合一输送带sfg - 2软自动上粉机 |
| 3. | In earlier cda works , transitivity in sfg was believed to be an efficient instrument for investigating ideologies hidden in news reporting
系统功能语法中的及物性在早期的批评性话语分析著作中被视为揭示新闻中隐藏的意识形态的有效工具。 |
| 4. | Halliday ’ s systemic - functional grammar ( sfg ) has been proven to be a scientific and practical theory . one of the most outstanding applications of this theory is discourse analysis
韩礼德系统功能语法的科学性及实用性已历经实践证明,其中最突出的应用是在语篇分析中发挥的巨大作用。 |
| 5. | The recombinant meq - baculovirus was obtained by co - transfecting the insect sf9 cells with pblubac4 - meq and linearised bac - n - blue dna . the recombinant baculovirus was selected by plaque assay and confirmed by pcr technique and sequencing of the inserted gene
应用重组痘病毒表达的meq制备的单抗23b46对重组杆状病毒感染的sfg细胞及其裂解物分别进行间接免疫荧光试验、 westemblot和免疫沉淀试验的检测。 |
| 6. | The angiostatin baculovirus transfer vector was co - transfected with viral dna into sf9 cells according to the manufacturers protocol . to purify the recombinant virus , we used the plaque assay to screen the pure recombinant plaque and amplify it to generate p - 1 stock
构建重组病毒：用已经构建好的angiostatin杆状病毒转移载体pbluebachiszb和病毒dna共同转染sfg细胞，通过蚀斑实验筛选出纯的重组斑点并扩增产生p二病毒贮存液。 |
| 7. | Meq protein , highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro . the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通过杆状病毒载体在昆虫细胞系sfg上高度表达的meq蛋白产物免疫balb / c小鼠，然后收获其免疫脾细胞并与肿瘤细胞系spz / 0通过peg于体外融合;获得的杂交瘤细胞被克隆并通过与mdv感染的鸡胚成纤维细胞( cef )做免疫荧光试验( fa ) ，进行其分泌抗meq单克隆抗体( mcab )能力的筛选。 |
| 8. | Once the recombinant virus has been i - dentified , we amplified the p - 1 stock to attain the large scale , high - titer viral stock in order to initiate expression studies . the recombinant angiostatin was produced from spodoptera frugiperda 9 ( sf9 ) insect cells infected by the high titer virus stock we have prepared . the time course for expression of recombinant protein was detected by sds - page and western blot , which can determine the optimal multiplicity of infection ( moi ) and the appropriate time of harvest for the protein
表达重组蛋白angiostatin ：用制备好的带有矗s标记的融合性angiostatin基因的高滴度重组杆状病毒贮存液感染草地贪夜蛾sfg细胞，用sds page电泳和hsternblot对感染不同时间后分泌的重组蛋白做时间表达分析，依此确定最适的感染复数（ moi ）和感染时间，以达到重组蛋白表达水平最适化，而后大规模进行重组蛋白的表达， sds page用来分析重组蛋白， westernblot用来在蛋白表达水平低的情况下检测表达的特异重组蛋白。 |
| 9. | Based on the result in 1 , through calculating the sfg parameter that induces the " green problem " , some theoretical conditions of suppressing green noises are put forward . after considering the feasibility of these conditions , some experimental schemes are brought forward . 3
在1的此基础上，通过对导致“绿光问题”的重要参量-和频( sfg )参数的计算，理论上得出了若干折叠腔内抑制绿光噪声的条件，在对这些条件进行可行性分析后，提出了若干抑制折叠腔-内腔倍频激光器绿光噪声的实验方案； 3 |
