Second , genomic dna polymorphisms of three mandarinfishes populations ( each bulked dna pool composed by 10 individuals ) were tested by rondom amplified polymorphic dna ( rapd ) using bulked segregant analysis ( bsa ) . 12 primers selected from operon kitz yielded reproducible and polymorphic dna fragments ranging from 125bp to 3kb . these primers generated 60 scorable marker bands of which 15 ( 25 % ) were polymorphic in qiupu river population ; 59 bands in changjiang population , 15 ( 25 . 4 % ) were polymorphic ; and 58 bands in wanfuhu mandarinfishes , 19 ( 32 . 8 % ) were polymorphic 20个引物中有12个在三群体间产生多态性，这12条引物在秋浦河鳜鱼中扩增出了60条带，其中15条为多态性(占25 ) ；长江鳜鱼59条带， 15条为多态性(占25 . 4 ) ；万佛湖鳜鱼58条带， 19条为多态性(占32 . 8 ) 。
