So far , a lot of data from field sero - epidemiological investigations and laboratory studies have demonstrated that the schistosome specific ige antibody may play an important role in defense against the infection 大量的血清流行病学资料和实验室研究的证据表明，血吸虫特异性ige抗体在抗血吸虫感染免疫中发挥重要作用。
2.
Under the guidance of the progressive theories and - 8 - understandings on the immunology and immunopathology of schistosome infection , seeking for the new vaccine - candidate molecules is also the important aspects of the vaccine research work 在对血吸虫感染免疫和免疫病理机制获得不断深入认识的基础上，继续寻找新的具免疫保扩作用的疫苗候选分子，是血吸虫病疫苗研究工作的重要内容。
3.
In this study , we at first aimed at obtaining the gene encoding the specific ige antibody related proteins by immunoscreening the schistosoma juponi cum adult worm cdna library with the pooled high - titer ige antisera from the individuals living in the schistosome epidemic regions J测定。 jnij序结果显示，该插入片段为1200hp ，第一开读框长507hp ，编码169个氨基酸残基，理论分子量为19 3kdi 。
4.
Rapid reinfection following treatment demands frequent retreatment , makes the chemotherapy approach expensive and chemotherapy cannot prevent reinfection . moreover , some schistosome strains of praziquantel - resistant or decreased susceptibility to the drug have been observed recently in several countries . therefore , it emphasizes the need for a more long - term approach 由于长期化疗存在需反复治疗、费用高以及不能预防再感染、有可能产生耐药虫株等缺点，所以寻找其他具有较长期效果的防治措施成为国内外专家学者关注的焦点，其中血吸虫病疫苗可能是一种费用较低而有效的措施。
5.
Yherefore , many researchers have shown great concern with the development of vaccines to against schistosomiasis . at early stage , the research of vaccines of schitosomiasis was centered on dead vaccine and athenuated cercaria of schistosomes while molecular vaccine and athenuated cercaria of schistosomes while molecular vaccine is currently the focus of research with anti - infection protective immunity as its main concern . guan xiaohong and zhao weixian ( 1986 ) certified that the allergen of egg granulonma of schistosoma japonicum might firstly come from gut associated antigen ( gaa ) of schistosomula and adult worm and that daa had cross reaction with soluble egg antigen ( sea ) and membrane associated antigen ( maa ) ; and the gaa of schistosoma japonicum might play a sensitizing role in egg granuloma formation Np30主动免疫c57bl 6对尾蚴攻击感染产生42 . 05的保护力，肝组织减卵率为66 . 63 ； balb c和昆明种小鼠的保护率分别为39 . 53和50 . 46 ；免疫山羊可诱导42 . 78的减虫率，肝组织减卵率为35 . 83 ，粪减卵率为25 ，并可明显抑制肝脏虫卵肉芽肿的大小，肉芽肿数量明显减少，纤维化减轻，体重明显增加，因此np30是南京医科大学博士学位论文很有希望的抗日本血吸虫病疫苗侯选分子。
6.
The target gene was then subcloned into plasmid vector and induced by iptg for its expression . after that , the recombinant protein was purified and used for the identification and characterization of its immunogenicity . the effect of the induced specific ige antibody on the hepatic granuloma formation and fibrosis after challenge infection with schistosome cercariae was evaluated Niptg诱导表达，产物沉淀中于约45kda处显见高合蛋白表达带， westernblot分析表明，该蛋白带可被筛库血清中特异性lge抗体识别；而载体本身表达的26gst蛋白带则否。
