Sonication - assisted agrobacterium - mediated transformation ( saat ) can increase the efficiency of transformation . 2s sonication can enhance the gus gene transient expression and induction of resistant buds without leaves incision 头孢毒素( cef )对不定芽的诱导有一定的抑制作用，哌拉西林钠( pip )对不定芽的诱导抑制作用稍弱，以pip400mg / l作为抑菌生长浓度。
