The pap gene was also transformed into wheat by pollen tube pathway - mediated and 320 grains of transgenic wheat was harvested . the seedlings of the seeds were inoculated with the gav isolate of barley yellow dwarf virus ( bydv ) by schizaphis graminum ( rondani ) and the results revealed that 33 wheat seedlings did not produce the symptom or the symptom was very mild 通过花粉管通道法将pap基因导入小麦,收获了转基因小麦种子320粒,将其播种,返青后,直接用麦二叉蚜将大麦黄矮病毒( bydv ) gav株系接种到幼苗叶片上,观察发病情况。