| 1. | Successive sampling survey ; retain sample ; matching ratio ; rhc sampling design 连续抽样保留样本匹配比rhc设计 |
| 2. | The board will decide whether a rhc should be appointed to hear the representations 城规会会决定应否委出一个聆讯申述小组委员会,聆讯申述个案。 |
| 3. | Each rhc consists of a chairman currently the director of planning , a deputy chairman a non - official member , two official members and five non - official members 每个聆讯申述小组委员会的成员包括1位主席目前为规划署署长1位副主席非官方成员两名官方成员及5名非官方成员。 |
| 4. | We also conducted the co - transfection with the rnai plasmids rhe and rhc and the selective marker gfp expressing plasmids simultaneously . there are three kinds of combination probability that could be observed by gfp marker 在实验中同时共转染人集缩素smc亚基hcap一e和hcap一c特异的rnai质粒rhe和rhc和pcdna3 . 1 + kg到hela细胞。 |
| 5. | This report explored the function of the human smc subunits - hcap - e and hcap - c using rnai and transfection techniques . we constructed two rnai plasmids - rhe and rhc that specifically corresponded to hcap - e and hcap - c of human condensin , respectively 为此我们构建了对人集缩素中两个smc亚基hcap - e和hcap - c特异的rnai质粒rhe和rhc 。 |
| 6. | The method for retaining sampled units in successive sampling survey for changed probability of selection is introduced . for pps sampling design , a model - design unbiased predictor for the total of a variable for the target population is proposed , and the optimum matching ratio for the predictor under the assumption of unchanged population is calculated . for rhc sampling design , the equation that the optimum matching ration satisfies is given 介绍了连续抽样中概率发生变化时保留样本的方法。对于有放回的pps抽样,在假设的超总体模型之下提出了总体变量总值的模型设计无偏预报量,并计算了总体不变时保留样本的最优匹配比。对于无放回的rhc抽样,给出了最优匹配比满足的方程。 |
| 7. | They are combination of co - transfection with rhe and gfp expressing plasmids , co - transfection with rhc and gfp expressing plasmids and co - transfection with plasmids rhe and rhc and gfp expressing plasmids at the same time . whichever the combination may be , we always observed the phenomena of chromatin bridge in co - transfected cells . these results indicate that the phenomena of chromatin bridge should be the characteristic feature of deficiency in the human smc subunits 这时,存在三种可能的情况,即共转染rhe和pedna3 . 1 * kc 、共转染rhc和pedna3 . 1 + kg 、共转染rhe和rhc和pc洲a3 . 1 + kg ,但都观察到明显的染色质桥现象,进一步证明这种表型是人集缩素中smc亚基的缺陷的特征表型。 |
| 8. | The individuals of rhd - positive phenotype with intact exons carried generally insert fragments and boxl box2 and box3 and this proved that inserts or rh box could n ' t affect the express of rh d gene . in 2 of the 5 wei nationality pedigrees whose proband were rh d - negative , rhc / e phenotype of all the rh negative individuals was ccee . rhd exon 4nsert and rh box did not be found in all individuals 在7个先证者为rhd阴性的汉族家系中,大部分成员均出现插入片段和rhbox ,且在遗传上符合孟德尔遗传定律, d外显子完整且表型为rhd阳性的家系个体成员广泛带有插入片段和box1 、 box2或box3 ,插入片段或rhbox并未影响d基因的表达。 |
| 9. | The effectiveness of the nuclear localization signal selected was clearly tested in initial experiments . based on this , the co - transfection systems used in the study was established . we first observed the multinucleate cells and chromatin bridges in cultured hela cells when the cells are marked with gfp and hochest33342 , after co - transfection with the gfp expression plasmids and rnai plasmids rhe or rhc 当分别共转染附加kozac序列和核定位信号的gfp与人集缩素smc亚基hcap一e特异的rnai质粒rhe和人集缩素smc亚基hca尸一c的f { nai质粒rhc时,都观察到gfp和hochest33342标记的转染后hela细胞表现多核和染色质桥现象。 |
| 10. | To study on structure and inheritance of rh d gene interaction between gene expression of rh d and rh c / e and influences on rh d gene expression of inserts and rh box - methods : 20 pairs of oligonucleotide specific primers for exon , intron 2 4 , insert and rh box of rh d , rhc / e gene were designed and composed the polymerase chain reaction - sequence specific oligonucleotide primer ( pcr - ssp ) was used to amplify the rh c / e gene , rh d gene , exon , intron , insert and rh box in 106 samples of unrelated individuals and 7 han nationality ancestries and 5 wei nationality ancestries whose proband were rh d - negative 目的:观察中国汉族非血缘关系随机个体、汉族及维吾尔族家系rh血型的c e基因、 d基因外显子、内含子、插入片段以及rhbox ,研究rhd基因结构及遗传规律, d基因表达与c e基因的关联,以及插入片段和rhbox对d基因表达的影响。 |