Chinese translation for "refold"
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- 再折叠
再摺起 重折叠
- Example Sentences:
| 1. | Refold along the crease , enclosing mixture , and make a double 1 / 2 - inch fold along the 3 open sides 依照折痕,再次折叠,包住食物,在其他三个开放面上也重复折叠两次,每次1 / 2英寸。 | | 2. | We have used two different operating schemes , fed - batch and step - addition , independently and collaboratively to refold denatured lysozymes 本研究中,我们利用馈料式与间歇式不同的复性方法,以独立地或结合地方式进行溶菌酶之复性。 | | 3. | This paper describe the formation , isolation , denaturation and renaturation of recombinant proteins as inclusion bodies in e . coli , and summarize the most efficient ways to refold recombinant proteins 摘要描述了大肠杆菌异源重组蛋白质的形成、制备、变性和复性,综述了国内外变性、复性的有效方法。 | | 4. | By the use of cirular dichroism and fluorescence techonologies to predict the secondary structure of protein , to determine the secondary structure of a protein , and to explore the unfold - refold mechanism of a protein 运用圆二色谱和荧光技术分析预测蛋白质二级结构,研究蛋白质的稳定性与蛋白质的变性复性动力学机制。 | | 5. | Section hi : purify & refold recombinant hpk - 5 protein was efficiently expressed in e . coli jm109 as inclusion bodies . after bacteria were smashed by ultrasound , te buffer , 1 % ttiton x - 100 and 2 m urea was used to efficiently extract inclusion bodies 用含sm尿素溶液洗涤包涵体, 8000r / min转速下分离包涵体,能最大限度去除杂蛋白,同时不会降低目的蛋白的损失。 | | 6. | We used gradient dilution renaluration ( renaturation buffer : 0 . 14mol / l tris - hcl ph8 . 0 . 10mmol / lgsh , 2mmol / lgssg . 5 % glycerol , 0 . 02mol / l l - arg ) and gel filter renaturation by sephacral s200 to refold the inclusion body respectively . to get pure nk4 , it was purified by gst affinity gromatochraphy . to detect the angiostatic activity of nk4 in vivo , it was tested using cam 6 )诱导温度对pg厂x一4t一l一nk4表达菌株bl21表达目的蛋自的影响分别进行了25 ’ c 、 300c 、 37温度状态卜,以iptg化学诱导,发现其均可表达,全菌表达量: 37最高,其次为30诱导时。 | | 7. | Mass spectrometry of synthetic hw - ma and rgd - hw are in full agreement with those speculated theoretically , which proves the success of peptide synthesis and refold . on isolated mouse phrenic nerve - diaphragm preparations , hw - ma can block the neuromuscular transmission in 35 minutes or so ( l 10 - 5 g / ml ) , its biological activity shows 73 % decrease comparing with biological activity of native hwtx - i . it proves t hat the protein engineering of synthetic chimera hwtx - i has gained success to some extent , although it did not achieve our expectations . thus it proved that hwtx - i can be using as natural scaffold for protein engineering . and also emphasized the importance of " local stereo circumstances " of activity site when the foreign activity site was transferred into a natural scaffold 浓度为1 / 1059 / ml的hw一ma突变体能可逆阻断小白鼠隔神经书高肌的接头传递,阻断时一间为35min左右,与天然hwtx一i比较,生物学活性下降3一4倍,说明合成的突变体改造获得了一定的成功,尽管与我们预期的目标有一定的差距,从而证明hwtx一i可以作为蛋白质工程研究的天然分子骨架,同时也强调了往天然分子骨架中转移外源活性位点时维持活性位点“局部立体环境”的重要性。 |
- Similar Words:
- "refocusing" Chinese translation, "refocusing method" Chinese translation, "refocusing the soul" Chinese translation, "refoios" Chinese translation, "refojo" Chinese translation, "refolded antiform" Chinese translation, "refolded fold" Chinese translation, "refolded limb" Chinese translation, "refolder" Chinese translation, "refolding" Chinese translation
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