| 1. | Protein kinase b ( pkb , also named kinase akt ) is newly found as a ser - ine / threonine protein kinase
Pkb akt2在卵巢癌、胰腺癌细胞中过表达， pkb akt3在大脑和睾丸中表达。 |
| 2. | The activity and expression of pkb increased in g2 / m phase , the mrna level also detectable contract to m ii oocytes
Pkb通过pi3k pkb途径调节细胞代谢、促进蛋白合成、细胞生长、增殖，另外还有抗细胞凋亡作用。 |
| 3. | Materials animal kunming genealogy mouse were provided by the laboatory animal department of china medical university . female with 4w ( 18g ) and male with 8w ( 30g )
Ly294002是pi3k的选择性抑制剂，已被广泛应用来阻断pi3k pkb信号转导通路，可作为pkb的抑制剂来观察其生物学功能。 |
| 4. | Correspondingly , the egf binding affinity of surface egfr and the tyrosine auto - phosphorylation of immuno - precipitated egfr were also enhanced in gntv - s / h7721 cells and reduced in gntv - as / h7721 cells
Egfr的信号通路主要有两条，一是ras - raf - mek一mapk经典途径，另一条是新发现的pi一3k一pkb途径。 |
| 5. | Cdc25 undergoes serine / thre - onine phosphorylation at mitosis and enhances its phosphatase activity . mpf phosphorylates and actives cdc25 , which in turn dephosphorylates and further activates prempf
Pkb分子量60kda ，目前已知分为pkb 、 、三种， pkb akt1广泛存在于机体各组织中，其活性受多种激活剂调节。 |
| 6. | Sodn oligodeoxynucleotide of pkb : 5cac atg gac gtc aaa gct tcc aa3 ' each base of both sodn and saodn oligodeoxynucleotide was sulfurated . methods 1 . superovulation , natural mating and eggs collection
Myt激酶的作用与cdc25相反，可以持续的使edcz的nlr14和t ”巧处于磷酸化状态而抑制mpf的活性，从而抑制细胞周期进程。 |
| 7. | Abtibidies goat anti - pkb polyclonal antibodies , antiphospho - tyr15 of cdc2 and antiphospho - ser216 of cdc25c polyclonal antibodies , alkaline phosphatase conjugated anti - goat secondary antibodies were purchased from santa cruz biotechnology
与edcz激酶增生细胞核抗原。一myt和c一myb基因互补的odn均可抑制平滑肌细胞的增生和移行。 |
| 8. | Both cdc2 and cdc25 kinase activity were inhibi - ted by ly294002 and saodn oligodeoxynucleotide of pkb , which indicate that pkb may act as an initiator of g2 / m phase transition through the phosphoryla - tion of cdc25c at ser216
Pi3k介导的信号转导通路中， pi3k pkb是独立于其它通路的一个新发现的信号转导通路，且与ras ， g蛋白， pkc等有复杂的交互作用。 |
| 9. | B - actin primers : forward 5tcc tat cgg cct cct cct ac 3 " and reverse 5aga atg tag tcc gcc agg tc 3 " , designed for 200bps . saodn oligodeoxynucleotide of pkb : stac agc ctg aga gga cct acc tg3 "
在有丝分裂期， edc25经历216位ser磷酸化同时其水解酶活性增强，活化的mpf磷酸化并活化edc25 ，形成正反馈，结果进一步活化mpf前体，活化的mpf促进细胞周期g2 / m期转换。 |
| 10. | There are two egfr signaling pathways , one is the classical ras / raf / mek / mapk pathway and the other is pi - 3 - k / pkb pathway . in this part , the phosphorylation and / or activity of some key molecules in these two signaling pathways in differently transfected h7721 cells were investigated . by means of specific antibodies combined with weste
Egf刺激后不同转染细胞中的pkb活性、 p42 / 44mapk和mek磷酸化都明显增加，其中gntv一s / h7721细胞pkb比活性、 p42 / 44mapk和mek磷酸化程度较mock细胞增加明显，而gntv一as / h7721细胞中上述指标的增加程度则不及mock细胞。 |
