Diehloromethyl biphenyl was synthesized through chloromethylation of biphenyl , starting with biphenyl and paraformaldehyde as raw materials , zinc chloride / h2o as catalyst in the presence of hydrogen chloride 摘要以联苯、多聚甲醛为起始原料，氯化锌h2o为催化剂，在氯化氢的存在下经氯甲基化反应，得到了4 , 4 ' -二氯甲基联苯。
2.
After administrated for successive 10 days , mice were anesthetized by sodium phenobarbital , carried out heart perfusion 30min with 2 % paraformaldehyde , then extracted hippocampus and submaxillary gland , made frozen sections 连续灌胃给药10天后，苯巴比妥钠麻醉小鼠，用2多聚甲醛心脏灌流固定30ndn ，取出海马和颌下腺，做冰冻切片。
3.
Material and methods normal rats of male sd were divided into young , adult , and aging groups . preparation of samples for light microscopy : animals were anesthetized by peritoneal injection of 6 % chloral hydrate ( 0 . 5ml / 100g body weight ) . perfusion and fixation of animals were carried out by a common procedure : 37 normal saline 50 - 100 ml and then 4 % paraformaldehyde pbs 100 - 400ml were perfused through the left ventricle of the heart , the whole procedure was lasted for about somin . the entire brain was dissected out and dipped in the fixative solution for 12h at 4 . brain pieces targeted were choosen and then passed the graded alcohols for dehydration , dipping into paraffin for embeding , and reshaping the pieces 2 ）磷酸缓冲液100400m ， 30分钟灌注完毕，取出整脑，在上述固定剂oc ）内后固定12小时。切取观察部位脑块，然后，进行梯度酒精脱水，浸蜡，包埋，修块，石蜡连续切片（德国leica石蜡切片机人切片厚度still ， zlllll ，蛋自甘油载片捞片， 60c烤箱过夜，二甲苯脱蜡，梯度酒精置换，浸水， h六染色，梯度酒精脱水，二甲苯透明，中性树脂封片。室温风干后，显微镜观片， olympus万能显微镜照相。
