Halobacillus trueperi accumulates glycine betaine under condition of high osmolarity . a partial fragment of the glycine betaine transporter beth gene was obtained from the genome of h , trueperi with degenerate primers . through southern blot hybridization and inverse pcr , a 5 . 1 kb ecori fragment containing the beth gene was sequenced 将扩增片段用地高辛标记成探针，与用不同限制性内切酶完全酶切的h . trueperi总dna片段作southern杂交，结果显示在ecori酶切片段的5 . 1kb处有阳性信号。
