Sds - page analysis revealed that lactococcus lactis nz9800 harbouring pmg36e / nisa restored little ability of nisin production 以lactococcuslactisnz9800为受体菌，研究了表达载体的表达情况。
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4 . the recombinant plasmid pmg36e / nisa was introduced into lactococcus lactis nz9800 . sds - page analysis revealed that lactococcus lactis nz9800 harbouring pmg36e / nisa restored no ability of nisin production 抽提ecolijm109中的重组质粒，转化l . lactisnz9800 ，在含红霉素的gm17平板上筛选转化子。
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3 . the amplified dna fragment was then ligated into the hindlll and ecori sites of pmg36e . the ligation mixture was transformed into jm109 for the initial cloning . the recombinant plasmid pmg36e / nisa isolated from jm109 transformants were analyzed by restriction enzyme digestion 将酶切并纯化后的pcr扩增产物与大片段连接并转化e . colijm109 ，在含红霉素的lb平板上筛选到含有重组质粒的转化子。
