| 1. | The signaling pathways involved in mediating pol p over - expression were also studied
Id与非定标性突变形成的关系以及mnng诱导p 。 |
| 2. | Therefore , we set out to explore the possible role of over - expressed pol p induced by mnng in untargeted mutagenesis
Lp可能作为增变因子参与了遗传不稳定的发生。因此我们在本课题中研究了p 。 |
| 3. | In this research , the expression of dna polymerase p was examined in mnng treated cells , where untargeted mutation had been proved arisen
Lp的表达：我们用western印迹方法检测了经mnng处理后细胞中的p 。 |
| 4. | Among them , fragment 9 showed enhanced expression after mnng exposure and protein synthesis inhibitor could n ' t inhibit the alteration
其中9号片段在mnng处理后表达增高，其表达改变不受蛋白质合成抑制剂的影响。 |
| 5. | In addition , more than 30 differential expressed sequence tags ( est ) have been isolated by mrna differential display
我们还利用mrna差异显示技术分离到了近30个在mnng处理后表达改变的表达序列标签（ expressedsequenceag ， est ） 。 |
| 6. | Time - course analysis showed that the frequency of mnng induced nontargeted mutation increased gradually , reached the peak at 12 h after mnng treatment , and then declined
且突变谱明显不同于由mnng直接攻击引起的定标性突变，有其突变好发部位的序列特异性。 |
| 7. | Our previous work identified delayed mutation occurred at target supf trna gene in plasmid transfected into cells pretreated with low concentration of alkylating agent n - methyl - n ' - nitro - n - nitrosoguanidine ( mnng )
在收回的质粒上我们检测到了延迟发生的、高于对照5倍以上的突变。由于w在细胞中的半寿期仅为1 |
| 8. | For example , we have found the clustering of egfr ( epidermic growth factor receptor ) and tnfr ( tumor necrosis factor receptor ) and the activation of camp - pka - creb and jnk / sapk pathways after mnng treatment
例如细胞表面受体如表皮生长因子受体、肿瘤坏死因子受体发生聚簇，细胞信号转导通路camp kacgyb和jnk sapk被激活。 |
| 9. | Direct damage on supf trna gene can be neglected because half - life of mnng is 1 . 1 hour and the interval between treatment and transfection was as long as 12 - 24 hours . therefore the mutagenesis is not lesion directed
20m的mnng经洗涤后的极微量残留经过10 ? 20多个半衰期的降解后，己不足攻击转人的qdna分子，因此这种突变显然是发生在mnng直接攻击部位以外的正常碱基上。 |
| 10. | Thus , we hypothesized that pol p might be involved in mnng induced untargeted mutagenesis . establishment of pol p down - regulated cell line : to verify the hypothesis , a human pol p down - regulated cell line was established using an antisense rna strategy
Lp表达阻断的细胞系：为验证此假设，我们用反义na技术3一i浙江大学博士学位论文王谷亮1建立了pcid表达下调的转基因细胞系。 |
