Moreover we have got the macroscopic binding constants of ktb - pro by monitoring fluorescence . independent of calcium ions , protein can also combine with melittin 而且，我们从中求得了tb ~ ( 3 + )和中心蛋白配合物的条件稳定常数。
2.
Megf and melittin was linked together by a linker into an chimeric dna , megfmel , and the chimeric dna was expressed by using pet30a as expression vector and e . coli as expression strain 值在slug砌l与54ug加l之间。在诱导表达各目的产物时诱导温度为30c 。
3.
The results indicated the spatial structure of the expressed product was correct , and the cationic region formed by two lys and two arg was necessary for keeping the activity of melittin 这些结果表明表达产物呈正确的空间折叠，而其中由2个lys和2个arg组成的正电区对melittin保持活性不可缺少。
4.
Melittin , which is taken as a typical cap , has many kinds of bioactivities . mouse epidermal growth factor , megf , as a factor to stimulate cell proliferation , also has been applied widely in clinical practice 小鼠表皮生长因子( mouseepidermalgrowthfactor , megf )作为一种致细胞增殖因子在临床上也有广泛应用。
5.
Mae designed by our lab is a hybrid peptide with ideal antibacterial activity . it derives from magaininii ( amino acid residues 4 - 14 ) and melittin ( amino acid residues 2 - 13 ) , and is optimized according to the relations between the structure and function Mae是本实验室设计的具有抗菌活性的杂合肽，它是以magainin (残基4 - 14 )和melittin (残基2 - 13 )为亲本，并对序列进行了优化改造后设计出的杂合肽。
6.
Cationic antibacterial peptides are found in insect , animal and plant . they are grouped into four classes including cecropins , magainins , melittins and defensins . cema consists of eight amino acid from n - terminus of cecropin a and c - terminus of modified melittin 阳离子抗菌肽是一类广泛存在于昆虫、动物、植物体内的小分子抗菌活性多肽，大体可分为：杀菌肽( cecropin ) 、蛙皮素( magainin ) 、蜂毒素( melittin ) 、防御素( defensin )等四大类。
7.
Abstract : the effects of terbium ion on the conformation of calmodulin and on the interaction between calmodulin and melittin have been studied by the endogenous fluorescent spectrometry of calmodulin and melittin , and the sensitized fluorescent spectrometry of terbium ion , respectively . the results show that terbium ions have a tight binding site in the i and ii metal - binding sites of calmodulin . the conformation of calmodulin induced by terbium ion can bind melittin and transfer the tryptophane residue of melittin to a relatively hydrophobic environment , while the binding of melittin to calmodulin produces effect on the binding orders of terbium ion in camodulin . results from ft - ir spectrometry have revealed that upon binding of lanthanum ion , apo - calmodulin undergoes a conformational change with the increase of - helix content and the decrease of - sheet content . melittin ' s binding to calmodulin has no effect on its conformation induced by the binding of lanthanum ion to calmodulin 文摘:分别用钙调蛋白和蜂毒素的内源荧光光谱以及铽离子的敏化荧光光谱考察了铽离子对钙调蛋白构象变化以及对钙调蛋白与蜂毒素相互作用的影响.结果表明，铽离子首先结合在钙调蛋白的第和第位点，铽离子不影响钙调蛋白与蜂毒素的相互作用，蜂毒素与钙调蛋白作用后不影响铽离子在钙调蛋白上的键合顺序.傅里叶变换红外光谱结果表明三价的镧离子与钙调蛋白作用使钙调蛋白的螺旋结构增加，折叠结构减少，与钙离子对它的二级结构影响相类似.稀土离子在钙调蛋白-蜂毒素复合体系中主要与钙调蛋白作用
8.
This project is aimed at : ( 1 ) expressing melittin and megf in e . coli respectively ; ( 2 ) constructing a membrane - toxic immunotoxin by using melittin as toxic part and megf as target part ; ( 3 ) measuring the megf mrna expression abundance by quantitative competitive rt - pcr ; ( 4 ) checking the genetic stability of the recombinant strains constructed in the project ; ( 5 ) optimizing the fermentation conditions of the target strains by flask shake 本课题以大肠杆菌( e . coli )宿主重组表达melittin和megf ；以melittin为毒性部分、 megf为靶向部分，构建膜毒性免疫毒素；用定量竞争rt - pcr法测定megf体内表达丰度；检验所构建的各重组菌株的遗传稳定性；采用摇瓶分析对各重组菌株的发酵条件进行了初步优化。
9.
The mature region cdna of melittin of apis cerana was cloned by using puct - vector developed from pucis vector . the sequence was compared with the corresponding region of melittin of apis mellifera reported in genbank , the mutant site was found in 33rd base ( a : apis mellifera ; g : apis cerana ) , but the primary structure of the two proteins were identical , which suggested melittin , as an important defensive gene , was very conservative in evolution 测序结果显示与意蜂( apismellifera ) melittin成熟区序列相比，中蜂( apiscerana ) melittin成熟区序列在第33位存在差异(意蜂为a ，中蜂为g ) ，但两者蛋白质一级结构相同，说明melittin作为蜜蜂一种重要的防御基因在进化上保守。
