From a cross 18 x 28 ( each from the superior tree from xianju county and pan " an county in zhejiang province ) . 236 random oligonucleotide primers were screened and 33 primers were selected to generate rapd markers within a sample of 60 megagametophyte dnas 通过236个10bp的随机引物或组合对6粒黄山松种子胚乳组织dna进行pcr扩增,共筛选出33个多态引物用于作图。