| 1. | One positive clone has been gained from the library screened by m4g3 mcab
3 .文库扩增后滴度达到1 |
| 2. | Mcab monoclonal antibody
单克隆抗体 |
| 3. | The average csfv titer of samples is 1 : 32 by indirect elisa , but 1 : 4 dilution is used in practice
通过方阵试验找出了mcab和血清多抗( pcab )的最佳工作稀释度。 |
| 4. | Sds - page and western - blot assay show that the expressed product was 29kda and could be recognized by anti - 6his mcab
Page和westernblot鉴定，获得原核表达的mbl蛋白。 |
| 5. | The ig class is iggi . mcab of at - iii provides very efficient tools for the detection and purification of at - iii
获得三株分泌抗人抗凝血酶的杂交瘤细胞株d5 、 e2 、 f6 ，免疫球蛋白的类型为igg _ 1型。 |
| 6. | After detection , 6 igm type and 4 igg1 type mcab were identified , and the all igg type mcab can capture xhfv antigen with antigen capture elisa but only 2 of the 6 igm mcab did
经检测， 10株上述单抗中， 4株属于igg1型， 6株属于igm型单抗； igg型单抗可全部用于捕捉xhf病毒抗原， igm型单抗不能全部用于捕捉xhf病毒抗原。 |
| 7. | A synthesised peptide which contain a partial sequence amino acid residues was used in i - elisa , the result is also positive , it means that the amino acid residues 28 - 35 of e2 protein is likely a linear epitope recognized by mcab all
从而证明核心序列能模拟a11所针对的抗原表位。研究结果提示单抗a11所针对的抗原表位位于e2蛋白的28 ? 35位氨基酸。该区域可能是csfve2一个新的抗原表位。 |
| 8. | Methods : sandwich elisa assay was used , w6 / 32 mcab serving as solid phase antibody and 3 2m antibody as the first antibody . the second antibody - hrp conjugate was added for coloration . standard curve was obtained by shla - i standard reagent in serial dilution . the amount of shla - i in the samples was determined : 1
方法：以w6 32包被酶标板，捕捉样品中可溶性hla ，加入一抗2m抗体，再加酶标二抗及底物显色。根据可溶性hla -的不同浓度标准品显色后的od值绘制标准曲线： 1 |
| 9. | Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides , we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library , which may be another form of antagonist for tnfa , and the mimotopes were identified by sandwich elisa . after 3 rounds of screening , we got 9 phage clones identified as positive clones which can bind with mcab j1d9 . we also identified the binding between mimotopes and tnf receptor by competitive elisa , and the results showed strongly binding . the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c
在对噬菌体环七肽库进行三轮亲和性筛选后，随机挑选20个噬菌体克隆， elisa鉴定出9个阳性克隆，经dna测序推出三种氨基酸序列： c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ，其中优势克隆序列为c - rrpaqsg - c ；鉴定结果显示阳性克隆能够与tnf受体结合，并且能够阻断tnf与受体的结合，提示筛选得到的环七肽克隆展示肽具有tnf的抗原性及与tnf受体结第一军医大学顾士学位论文合的特性，为tnfa表位模拟肽。 |
| 10. | In this experiment , to screen the epitope of e2 protein of classical swine fever virus ( csfv ) defined by the monoclonal antibody ( mcab ) all , which had been prepared in previous experiment , the mcab all was raised in mice and followed by purification , the concentration of protein was assayed by using the bca protein assay kit
本室利用e2基因疫苗制备了多株单抗，为e2的抗原表位研究提供了条件，我们以噬菌体随机12肽库分析鉴定了猪瘟病毒e2蛋白的抗原表位，为深入研究猪瘟病毒的抗原结构、制备更有效的诊断试剂和疫苗提供更多理论依据。 |
