| 1. | Research progress and application prospect of magnetosome
细菌磁小体形成机制和应用研究进展 |
| 2. | It indicated that the magnetosome deleted phenotype w as caused by tn5 insertion
Southern杂交验证磁小体缺失突变确为tn5插入所致。 |
| 3. | Progress in genomic study and biochemical formation of magnetosome chains in magnetotactic bacteria
趋磁细菌基因组和磁小体成链机制研究进展 |
| 4. | It was demonstrated that this fragment was related to magnetosome biosynthesis by experiment of functional complement
功能互补实验证明其与磁小体合成相关。 |
| 5. | Two magnetosome deletion mutants were constructed by conjugative transposon mutagensis and the application of this genetic system . the two magnetosome deletion mutants were named as nm4 and nm21 respectively
并利用此体系，通过接合转座诱变技术，获得了2个磁小体缺失突变株： nm4 、 nm21 。 |
| 6. | The sequences flanking tn5 in magnetosome deleted mutant nm4 was cloned by anchored pcr , and was used as tag to extend the 5 ' and 3 ' terminal sequences of the target dna fragment by anchored pcr constinuously
以tn5为标签，用锚定pcr法克隆出突变株nm4的tn5侧翼序列。并以此侧翼序列为标签继续用锚定pcr法向两边延伸，克隆出一个5045bp的dna片段。 |
| 7. | The tn5 inserted in the orf4 . it was demonstrated that this fragment was related to magnetosome biosynthesis by experiment of functional complement . the orf4 encodes a protein which is homologous to the atpase from magnetospirillum magnetotacticum ms - 1 and contains a rec domain which has a chey - homologous receiver domain
该片段由6个orf组成， tn5插入在3621bp - 3622bp间，位于orf4起始密码子下游的84bp处， orf4因tn5插入失活，通过功能互补实验证明该片段与磁小体合成相关。 |
| 8. | The protein encoded by orf1 show strong homology with cation efflux family protein from geobacter sulfurreducens pca [ identities = 65 / 272 ( 23 % ) ] , and contains mmt1 - like putative conserved domains kog1485 , mmt1 contains conserved domains kog1485 is mitochondrial fe2 + transporter ( cation diffusion facilitator superfamily ) . so it can be considered that the protein encoded by o rf1 is fe2 + transporter involved in magnetosome biosynthesis in magnetospirillum gryphiswaldense msr - 1
经同源比较和保守性结构域分析，推测orf1编码的蛋白具有fe ~ ( 2 + )转运功能，在磁小体合成过程中将细胞质中fe ~ ( 2 + )转运到磁小体囊泡中，合成磁小体的同时去除fe ~ ( 2 + )对细胞的毒害作用。 |
| 9. | This domain receives the signal from the sensor partner in bacterial two - component systems . these results indicated that the protein of the orf4 works as atpase function involved in the synthesis of magnetosome or takes part in the signal transduction relate to the promotion of magnetosome biosynthesis
通过对orf4编码蛋白同源比较和功能分析，推测orf4编码蛋白可能的功能有两种：具atpase活性，在磁小体合成过程中为铁转运提供能量；或参与磁小体合成启动过程中的信号转导。 |
| 10. | The genetic analysis of the mechanism of magnetosome biosynthesis in magnetospirillum gryphiswaldense msr - 1 has been hampered by the lack of an appropriate genetic manipulation system . here reported on the establishment of a genetic manipulation system that can be used more readily than that of the others for magnetospirillum
格瑞菲斯瓦尔德磁螺菌( magnetospirillumgryphiswaldensemsr - 1 )为趋磁螺菌属( magnetospirillum )的模式菌株，能够在胞内合成大小约为42am的磁小体( magnetosome ) 。 |
