Influence of 3 ' untranslated region single nucleotide polymorphism of tlr4 gene on luciferase gene expression 位点突变对荧光素酶表达的影响
2.
Construction of adenoviral vector for luciferase driven by htert core promoter modified with myc - responsive elements 核心启动子引导荧光素酶表达的腺病毒载体
3.
The inhibition of prl on luciferase gene expression in the group of transfected cho cells was observed as pgl3 - l . 6 > pgl3 - 2 . 5 > pgl3 - 338 证明叩l和hcg的调控元件主要位于20ahsd基因的15s7 286之间。
4.
Further , their luciferase activities were determined in ty medium , and showed that tn5 - 1063 was inserted directly into loci downstream of promoters in 042bm genome 它们都表现发光酶活性,表明转座子正向插入到基因组中的某个启动子下游。
5.
Tumor necrosis factor ( tnf ) , a cytkine that exerts many pro - atherosclerotic effects in vivo , causes up - regulation of acat - 1 gene expression in human blood monocytes . by luciferase activity assay , tnf - a enhanced acat - 1 p7 promoter activity in thp - 1 monocytic cell line 细胞因子tnf -即肿瘤坏死因子大量存在于人动脉粥样硬化斑块中,本实验通过荧光素酶活性和rt - pcr测定结果表明, tnf -处理人血单核细胞和单核细胞株thp - 1均增强acat - 1基因p7启动子活性。
6.
To test the p7 promoter activity , a series of constructs were obtained by cloning the different dna fragments into the luciferase gene reporter vector pgl3 - b . when the constructs were transiently transfected into thp - 1 cells , luciferase activity assay showed that the core region of p7 promoter located at - - 165 bp 第一部分的工作首先通过对人acat - 1基因p7启动子序列进行5 '和3 ' -端的缺失的详细分析,结果显示最大报告基因转录表达活性位于- 612 - 165bp区段。