| 1. | Cloning and analysis of lfy - like gene from citrus
1同源基因的克隆和序列分析 |
| 2. | 2 % , which is 9 . 8 % lower than lfy and flo ( 70 % )
2 ，这小于被子植物拟南芥lfy与金鱼草flo之间的同源性（ 7 ） 。 |
| 3. | Ginndly ( male ) amino sequence similarity with lfy , prfll ( pinus ) , needly ( pinus ) is 45
3和58二；与辐射松needly同源性分别为58二和75石。 |
| 4. | Ginlfy ( male ) amino sequence similarity with lfy , prfll ( pinus ) , needly ( pinus ) is 50 . 0 % , 80 . 3 % and 58 . 2 % , respectively
另外，与拟南芥l厂y同源性分别为50刀和扔：与辐射松fll同源性分别为80 |
| 5. | The intron of the gene have similar characteristics with the lfy like of many plant species . the gene contain two introns and three exons
从内含子边界特征和多种植物leafy基因同源性比较，确定了该基因的内含子位置。 |
| 6. | Leafy ( lfy ) , one of floral meristem identity genes , which confers transition from vegetative to floral meristem identity in arabidopsis , and affects its flowering time . overexpression lfy gene promotes plant earlier flowering
其中leafy ( lfy )是一个花分生组织特征基因，控制着拟南芥营养分生组织向花分生组织的转变，也影响拟南芥的开花时间。 |
| 7. | Many studies show that leafy is high homolog even among distantly related plant species . exception of these , little studies on tissue culture and transformation of ginkgo have been done . this paper emphasizes on the isolation , cloning and analysing two ginkgo orthologs of leafy from the male tree
为此，本实验从银杏leafy同源基因的克隆入手，分析其雌雄株lfy基因结构差异，构建lfy基因的植物正义反义表达载体，建立矮牵牛遗传转化体系，以研究银杏lfy同源基因的功能，同时建立了银杏组织培养体系，为银杏的遗传转化和提早开花结果奠定基础。 |
| 8. | Lfy and lfy - like proteins from angiosperm contain a proline - rich region near the amino terminal and an acidic central region which is typical for transcriptional activators and may be important for the function of lfylike protein . the two domain exists in the variable regions of all lfy - like proteins
与拟南芥leaf ’ y基因相比较，银杏ginlfy和g毗基因没有做为转录激活区域的位于氨基酸序列中间的酸性区和位于n端的富含脯氨酸区，但这两个区域均位于leafy基因的非保守区。 |
| 9. | The phylogenic relation and similarity of the gene is full length of lfy - like gene , which is 99 % similar both in nucleic acid sequence and amino acid sequences with that of ginlfy f rom the female . compared with the ginlfy of female , the gene from the male tree is deficient in 3 bp , and mutantion sites exists on the varied region of lfy - like
该基因与文献报道的银杏雌株leafy基因相比，核苷酸序列同源性高达99 ，蛋白质序列同源浙江大学博士学位论文摘要性达99 ，缺少了3个核昔酸，突变均在植物leaf ’ y基因的非保守区内。 |
